Integration Host Factor IHF facilitates homologous recombination and mutagenic processes in Pseudomonas putida
| Autor: | Heili Ilves, Kärt Ukkivi, Maia Kivisaar, Katren Mikkel, Mari Tagel |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Integration Host Factors
Biology Biochemistry Frameshift mutation 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Plasmid Bacterial Proteins Point Mutation Frameshift Mutation Homologous Recombination Molecular Biology 030304 developmental biology Genetics 0303 health sciences Pseudomonas putida Point mutation Chromosome Cell Biology Chromosomes Bacterial biology.organism_classification biological factors chemistry 030220 oncology & carcinogenesis bacteria Homologous recombination Recombination DNA Plasmids |
| Zdroj: | DNA repair. 85 |
| ISSN: | 1568-7856 |
| Popis: | Nucleoid-associated proteins (NAPs) such as IHF, HU, Fis, and H-NS alter the topology of bound DNA and may thereby affect accessibility of DNA to repair and recombination processes. To examine this possibility, we investigated the effect of IHF on the frequency of homologous recombination (HR) and point mutations in soil bacterium Pseudomonas putida by using plasmidial and chromosomal assays. We observed positive effect of IHF on the frequency of HR, whereas this effect varied depending both on the chromosomal location of the HR target and the type of plasmid used in the assay. The occurrence of point mutations in plasmid was also facilitated by IHF, whereas in the chromosome the positive effect of IHF appeared only at certain DNA sequences and/or chromosomal positions. We did not observe any significant effects of IHF on the spectrum of mutations. However, despite of the presence or absence of IHF, different mutational hot spots appeared both in plasmid and in chromosome. Additionally, the frequency of frameshift mutations in the chromosome was also strongly affected by the location of the mutational target sequence. Taking together, our results indicate that IHF facilitates the occurrence of genetic changes in P. putida, whereas the location of the target sequence affects both the IHF-dependent and IHF-independent mechanisms. |
| Databáze: | OpenAIRE |
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