Transcription factor activity and nucleosome organization in mitosis
| Autor: | Nicola Festuccia, Alexandra Tachtsidi, Nancy Gutierrez, Elena Gallego, Agnès Dubois, Thaleia Papadopoulou, Inma Gonzalez, Sandrine Vandoermel-Pournin, Pablo Navarro, Nick D.L. Owens, Michel Cohen-Tannoudji |
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| Přispěvatelé: | Epigénétique des Cellules Souches - Epigenetics of Stem Cells, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique ( CNRS ), Sorbonne Université ( SU ), Génétique fonctionnelle de la Souris, This work was supported by recurrent funding from the Institut Pasteur, the CNRS, and Revive (Investissement d’Avenir, ANR-10-LABX-73). P.N. acknowledges financial support from the Fondation Schlumberger (FRM FSER 2017), the Agence Nationale de la Recherche (ANR 16 CE12 0004 01 MITMAT), and the Ligue contre le Cancer (LNCC EL2018 NAVARRO). N.F was supported by a Marie Curie IEF fellowship and a Pasteur-Cantarini Fellowship program. N.O. is supported by Revive., ANR-10-LABX-0073/10-LABX-0073,REVIVE,Stem Cells in Regenerative Biology and Medicine ( 2010 ), ANR-16-CE12-0004,MitMAT,Mémoire mitotique de l'activité transcriptionnelle dans les cellules ES ( 2016 ), Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], Sorbonne Université (SU), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), We thank the Imagopole France–BioImaging infrastructure, supported by the French National Research Agency (ANR 10-INSB-04-01, Investments for the Future), for advice and access to the UltraVIEW VOX system. We also thank the Transcriptome and EpiGenome, BioMics, Center for Innovation and Technological Research of the Institut Pasteur for NGS. We thank Elphège Nora and Benoit Bruneau for sharing reagents and advice to establish the auxin-dependent degradation system., ANR-10-LABX-0073,REVIVE,Stem Cells in Regenerative Biology and Medicine(2010), ANR-16-CE12-0004,MitMAT,Mémoire mitotique de l'activité transcriptionnelle dans les cellules ES(2016), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Předmět: |
Nucleosome organization
Cell division [SDV]Life Sciences [q-bio] Succinimides [SDV.BC]Life Sciences [q-bio]/Cellular Biology [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] Biology mitotic bookmarking Fixatives Mice 03 medical and health sciences 0302 clinical medicine SOX2 Formaldehyde [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] Genetics Animals Nucleosome [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology [ SDV.BDD ] Life Sciences [q-bio]/Development Biology Enhancer [SDV.BDD]Life Sciences [q-bio]/Development Biology Mitosis Transcription factor [ SDV.BBM ] Life Sciences [q-bio]/Biochemistry Molecular Biology Cells Cultured Genetics (clinical) transcription factor 030304 developmental biology mitosis 0303 health sciences fixation [ SDV ] Life Sciences [q-bio] Bookmarking [ SDV.BC ] Life Sciences [q-bio]/Cellular Biology Research nucleosome [ SDV.BC.BC ] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] Chromosomes Mammalian Chromatin Nucleosomes Cell biology [SDV.BDD.EO]Life Sciences [q-bio]/Development Biology/Embryology and Organogenesis Receptors Estrogen [ SDV.BBM.GTP ] Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] [ SDV.BDD.EO ] Life Sciences [q-bio]/Development Biology/Embryology and Organogenesis 030217 neurology & neurosurgery Transcription Factors |
| Zdroj: | Genome Research This article is a preprint: it has not been peer-reviewed. 2018 Genome Research, Cold Spring Harbor Laboratory Press, 2019, 29 (2), pp.250-260. ⟨10.1101/392241⟩ Genome Research, 2019, 29 (2), pp.250-260. ⟨10.1101/392241⟩ |
| ISSN: | 1549-5469 1088-9051 |
| DOI: | 10.1101/gr.243048.118 |
| Popis: | This article is a preprint: it has not been peer-reviewed.; Mitotic bookmarking transcription factors (BFs) maintain the capacity to bind to their targets during mitosis, despite major rearrangements of the chromatin. While they were thought to propagate gene regulatory information through mitosis by statically occupying their DNA targets, it has recently become clear that BFs are highly dynamic in mitotic cells. This represents both a technical and a conceptual challenge to study and understand the function of BFs: first, formaldehyde has been suggested to be unable to efficiently capture these transient interactions , leading to profound contradictions in the literature; second , if BFs are not permanently bound to their targets during mitosis, it becomes unclear how they convey regulatory information to daughter cells. Here, comparing formaldehyde to alternative fixatives we clarify the nature of the chromosomal association of previously proposed BFs in embryonic stem cells: while Esrrb can be considered as a canonical BF that binds at selected regulatory regions in mitosis, Sox2 and Oct4 establish DNA sequence independent interactions with the mitotic chromosomes, either throughout the chromosomal arms (Sox2) or at pericen-tromeric regions (Oct4). Moreover, we show that ordered nu-cleosomal arrays are retained during mitosis at Esrrb book-marked sites, whereas regions losing transcription factor binding display a profound loss of order. By maintaining nucleosome positioning during mitosis, Esrrb might ensure the rapid post-mitotic re-establishment of functional regulatory complexes at selected enhancers and promoters. Our results provide a mech-anistic framework that reconciles dynamic mitotic binding with the transmission of gene regulatory information across cell division. mitotic bookmarking | mitosis | nucleosome | transcription factor | fixation Correspondence: pnavarro@pasteur.fr |
| Databáze: | OpenAIRE |
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