Cystic fibrosis and the relationship between mucin and chloride secretion by cultures of human airway gland mucous cells
| Autor: | Lorna Zlock, Anna Y. Lao, Vijay Dasari, Walter E. Finkbeiner, Jonathan Widdicombe, Masatoshi Morikawa |
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| Rok vydání: | 2011 |
| Předmět: |
Adult
Pulmonary and Respiratory Medicine Respiratory Mucosa Pathology medicine.medical_specialty Exocrine gland Cystic Fibrosis Physiology Primary Cell Culture Cystic Fibrosis Transmembrane Conductance Regulator Bronchi Biology Cystic fibrosis Bronchoconstrictor Agents Phenylephrine Adenosine Triphosphate Exocrine Glands Chlorides Physiology (medical) Internal medicine medicine Humans ortho-Aminobenzoates Methacholine Chloride Ion Transport Mucin Isoproterenol Mucins Articles Cell Biology Human airway Adrenergic beta-Agonists Calcium Channel Blockers medicine.disease Mucus Cystic fibrosis transmembrane conductance regulator Trachea Endocrinology medicine.anatomical_structure Cell culture biology.protein Adrenergic alpha-1 Receptor Agonists |
| Zdroj: | American Journal of Physiology-Lung Cellular and Molecular Physiology. 301:L402-L414 |
| ISSN: | 1522-1504 1040-0605 |
| Popis: | —We investigated how cystic fibrosis (CF) alters the relationship between Cl−and mucin secretion in cultures of non-CF and CF human tracheobronchial gland mucous (HTGM and CFTGM, respectively) cells. Biochemical studies showed that HTMG cells secreted typical airway mucins, and immunohistochemical studies showed that these cells expressed MUC1, MUC4, MUC5B, MUC8, MUC13, MUC16, and MUC20. Effects of cumulative doses of methacholine (MCh), phenylephrine (Phe), isoproterenol (Iso), and ATP on mucin and Cl−secretion were studied on HTGM and CFTGM cultures. Baseline mucin secretion was not significantly altered in CFTGM cells, and the increases in mucin secretion induced by mediators were unaltered (Iso, Phe) or slightly decreased (MCh, ATP). Across mediators, there was no correlation between the maximal increases in Cl−secretion and mucin secretion. In HTGM cells, the Cl−channel blocker, diphenylamine-2-carboxylic acid, greatly inhibited Cl−secretion but did not alter mucin release. In HTGM cells, mediators (10−5M) increased mucin secretion in the rank order ATP > Phe = Iso > MCh. They increased Cl−secretion in the sequence ATP > MCh ≈ Iso > Phe. The responses in Cl−secretion to MCh, ATP, and Phe were unaltered by CF, but the response to Iso was greatly reduced. We conclude that mucin secretion by cultures of human tracheobronchial gland cells is independent of Cl−secretion, at baseline, and is unaltered in CF; that the ratio of Cl−secretion to mucus secretion varies markedly depending on mediator; and that secretions induced by stimulation of β-adrenergic receptors will be abnormally concentrated in CF. |
| Databáze: | OpenAIRE |
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