Liquid chromatographic determination of sulindac and metabolites in serum
| Autor: | William R. Ravis, Campagna Kd, Clark Cr, McMillian Cl, Hoke Jf |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Sulindac
Chromatography Resolution (mass spectrometry) Nitrile Metabolite Extraction (chemistry) General Medicine High-performance liquid chromatography digestive system diseases Analytical Chemistry chemistry.chemical_compound chemistry Indenes medicine Humans Gas chromatography Acetonitrile Biotransformation Chromatography High Pressure Liquid medicine.drug |
| Zdroj: | Journal of chromatographic science. 25(6) |
| ISSN: | 0021-9665 |
| Popis: | An improved liquid chromatographic procedure is described for the quantitative determination of sulindac, sulindac sulfone, and sulindac sulfide from serum. The procedure makes use of acetonitrile extraction of the compounds of interest from acidified serum samples. Under these conditions extraction efficiencies in the 85 percent range are obtained for each of the compounds. The liquid chromatographic separation of the compounds of interest and the internal standard (indomethacin) is accomplished in an isocratic elution procedure using a nitrile (CN) stationary phase. The HPLC separation procedure is completed in less than 10 minutes, giving excellent resolution and peak shape. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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