A membrane antigen of rabbit thymus cells
| Autor: | D.P. Fradelizi, Bernhard Cinader, C.-T. Chou, S. Dubiski |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
T-Lymphocytes
Immunology Bone Marrow Cells Hemolytic Plaque Technique Spleen Thymus Gland Viral Plaque Assay Cell Fractionation Adjuvants Immunologic Antigen Bone Marrow Isoantibodies medicine Animals Antigens Cytotoxicity Lymph node Antiserum biology Goats Immune Sera Cell Membrane Cytotoxicity Tests Immunologic Virology Molecular biology Allotype medicine.anatomical_structure Antibody Formation biology.protein Lymph Nodes Rabbits Bone marrow Antibody Thymidine |
| Zdroj: | Cellular Immunology. 7:484-501 |
| ISSN: | 0008-8749 |
| Popis: | Rabbit cells, bearing a thymus-specific antigen, which we call rabbit thymus lymphocyte antigen (RTLA), could be detected with a suitably absorbed heterologous antiserum (goat). In the presence of complement, the RTLA antiserum lysed more than 95% of thymus cells, 70 ± 6% of lymph node cells, 46 ± 10% of spleen cells and 12 ± 7% of bone marrow cells. The number of direct or indirect hemolytic spleen plaques was not reduced by treatment with RTLA antiserum and complement, but was greatly diminished by an unabsorbed thymus antiserum which killed more than 90% of bone marrow cells. RTLA-bearing subpopulations of spleen cells were characterized by velocity sedimentation analysis and were distinguished from Ig receptor bearing subpopulations. The antiserum concentration could be so adjusted that the cytotoxicity against bone marrow was not manifested, while the cytotoxicity against other cell populations remained unchanged. The latter were identified by thymidine incorporation induced by treatment with antibody directed against rabbit light chain allotype. A small subpopulation of thymus cells did not have RTLA antigen and sedimented with a velocity distinct from that of the peak of RTLA-bearing cells. |
| Databáze: | OpenAIRE |
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