A comparison of 24 chemicals in the six-well bacterial reverse mutation assay to the standard 100-mm Petri plate bacterial reverse mutation assay in two laboratories

Autor: John Nicolette, Alison Kondratiuk, G. Jayakumar, Paul Sonders, Meredith Crosby, A. Patel, Emily Dakoulas, Joel Murray, Kamala Pant, R. Vicente, K. Datta, M. Mathur, Rohan Kulkarni, Kyle L. Kolaja
Rok vydání: 2018
Předmět:
Zdroj: Regulatory toxicology and pharmacology : RTP. 100
ISSN: 1096-0295
Popis: The bacterial reverse mutation assay (Ames) is a fundamental genetic toxicology test, and efforts to miniaturize the regulatory GLP version are essential in assessing genotoxic liabilities earlier in the drug development pipeline. Two versions of the Ames were compared: the six-well (miniaturized) plate and the standard 100-mm plate test at two different laboratories. Of twenty-four chemicals tested, a subset of six chemicals was tested in the six-well test only and the remaining eighteen were evaluated in both versions of the test. The plate incorporation procedure was used with one Escherichia coli and four different Salmonella strains. The six-well test uses the same plating procedure and evaluation methods as the standard Ames assay in 100-mm plates, but the smaller format requires 20% of the test chemical. Additionally, the six-well test uses a limit concentration of 1000 μg/well versus the standard Petri plate test limit concentration of 5000 μg/plate. Testing across the two formats resulted in 100% concordance in overall mutagenicity judgement and 94% concordance across all tester strains and conditions. Known mutagenic positive control chemicals were correctly detected as positive in both formats. The overall conclusion is that the six-well assay results are concordant with the standard assay format in this evaluation and could be a reliable alternative.
Databáze: OpenAIRE
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