Effect of Overexpression of Actinobacillus succinogenes Phosphoenolpyruvate Carboxykinase on Succinate Production in Escherichia coli

Autor:	Pil Kim, Claire Vieille, J. Gregory Zeikus, Maris Laivenieks
Rok vydání:	2004
Předmět:	endocrine system endocrine system diseases Mutant Succinic Acid medicine.disease_cause digestive system Applied Microbiology and Biotechnology Escherichia coli medicine Ecology biology nutritional and metabolic diseases Actinobacillus Physiology and Biotechnology biology.organism_classification Enterobacteriaceae Phosphoenolpyruvate Carboxylase Culture Media Actinobacillus succinogenes Biochemistry Fermentation Mutation Genetic Engineering Phosphoenolpyruvate carboxykinase Phosphoenolpyruvate carboxylase hormones hormone substitutes and hormone antagonists Phosphoenolpyruvate Carboxykinase (ATP) Food Science Biotechnology
Zdroj:	Applied and Environmental Microbiology. 70:1238-1241
ISSN:	1098-5336 0099-2240
Popis:	Succinate fermentation was investigated in Escherichia coli strains overexpressing Actinobacillus succinogenes phosphoenolpyruvate carboxykinase (PEPCK). In E. coli K-12, PEPCK overexpression had no effect on succinate fermentation. In contrast, in the phosphoenolpyruvate carboxylase mutant E. coli strain K-12 ppc :: kan , PEPCK overexpression increased succinate production 6.5-fold.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6231d83a41a45d0d73f44e299ccfe29d https://doi.org/10.1128/aem.70.2.1238-1241.2004 Zobrazit plný text záznamu