A micromethod for the enzymatic estimation of the degree of glycogen ramification
| Autor: | M.T. Serafini, Marià Alemany |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Male
Formic acid Biophysics Polysaccharide Biochemistry Anthrone chemistry.chemical_compound Hydrolysis Mole Carbohydrate Conformation Animals Ethanol precipitation Anthracenes chemistry.chemical_classification Chromatography Glycogen Microchemistry Periodate Rats Inbred Strains Enzymes Liver Glycogen Rats Glucose chemistry |
| Zdroj: | Journal of Biochemical and Biophysical Methods. 15:33-39 |
| ISSN: | 0165-022X |
| DOI: | 10.1016/0165-022x(87)90060-1 |
| Popis: | A comparison of methods for the evaluation of glycogen content in liver tissue of rats has been carried out by determining the recoveries in the differential ethanol precipitation of glycogen from alkaline tissue digests as well as the actual quantitative equivalence between glycogen content and actual glucose measured. Hydrolytic/enzymatic methods gave lower results than non-specific chemical methods such as anthrone. These lower values, combined with the losses in the purification process resulted in much lower glycogen estimations than the actual estimated tissue content. A method has been devised for the measurement of glycogen ramification in small liver tissue samples, using neutral periodate oxidation of the molecule, followed by determination of the formic acid evolved from the branch ends with formic acid dehydrogenase. The method gave results very similar to the classical methods in which the acid formed is measured titrimetically. Rat liver tissue contained a mean 323 ± 69 mmol of glucose equivalents of glycogen per gram of tissue; this glycogen had a mean chain length of 11.4 ± 0.8 units. |
| Databáze: | OpenAIRE |
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