A collagen hydrolysate/milk protein-blend stimulates muscle anabolism equivalently to an isoenergetic milk protein-blend containing a greater quantity of essential amino acids in older men
| Autor: | Daniel J. Wilkinson, Matthew S. Brook, Paula J. Scaife, B.E. Phillips, Jessica Cegielski, Joseph J. Bass, S. Watanabe, Philip J. Atherton, Kenneth Smith |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male NEAA non-essential amino acids Calorie Anabolism Protein Hydrolysates FED feed BMI body mass index Muscle Proteins Critical Care and Intensive Care Medicine PVDF polyvinylidene difluoride 0302 clinical medicine Protein biosynthesis Medicine Insulin CP collagen protein hydrolysate Amino Acids 1-RM 1 repetition maximum chemistry.chemical_classification Nutrition and Dietetics Cross-Over Studies Milk Proteins Amino acid medicine.anatomical_structure Original Article Collagen Leucine MPB muscle protein breakdown Signal Transduction medicine.medical_specialty 030209 endocrinology & metabolism Oral nutritional supplement Muscle protein synthesis Hydrolysate TBST tris buffered saline/Tween 20 03 medical and health sciences Internal medicine ONS oral nutritional supplement Humans MP milk protein Muscle Skeletal EAA essential amino acids Exercise Aged Food Formulated Essential amino acids 030109 nutrition & dietetics business.industry DIAAS digestible indispensable amino acid score FED-EX feed + exercise Skeletal muscle MPS muscle protein synthesis Ageing BCAA branched chain amino acids Endocrinology chemistry VL vastus lateralis Dietary Supplements Amino Acids Essential business |
| Zdroj: | Clinical Nutrition (Edinburgh, Scotland) |
| ISSN: | 1532-1983 |
| Popis: | Summary Background & aims Nutritional composition is key for skeletal muscle maintenance into older age. Yet the acute effects of collagen protein blended with other protein sources, in relation to skeletal muscle anabolism, are ill-defined. We investigated human muscle protein synthesis (MPS) responses to a 20 g blend of collagen protein hydrolysate + milk protein (CP+MP, 125 ml) oral nutritional supplement (ONS) vs. 20 g non-blended milk protein source (MP, 200 ml) ONS, in older adults. Methods Healthy older men (N = 8, 71±1 y, BMI: 27±1 kg·m−2) underwent a randomized trial of 20 g protein, from either a CP+MP blend (Fresubin®3.2 kcal DRINK), or a kcal-matched (higher in essential amino acids (EAA) ONS of MP alone. Vastus lateralis (VL) MPS and plasma AA were determined using stable isotope-tracer mass spectrometry; anabolic signaling was quantified via immuno-blotting in VL biopsies taken at baseline and 2/4 h after ONS feeding. Plasma insulin was measured via enzyme-linked immunosorbent assay (ELISA). Measures were taken at rest, after the feed (FED) and after the feed + exercise (FED-EX) conditions (unilateral leg exercise, 6 × 8, 75% 1-RM). Results MP resulted in a greater increase in plasma leucine (MP mean: 152 ± 6 μM, CP+MP mean: 113 ± 4 μM (Feed P |
| Databáze: | OpenAIRE |
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