Strigolactone biosynthesis is evolutionarily conserved, regulated by phosphate starvation and contributes to resistance against phytopathogenic fungi in a moss, Physcomitrella patens
| Autor: | Mikko T. Lehtonen, Lina Baz, Eva L. Decker, Stefan Hunn, Jenny Ferguson, Steffen Nordzieke, Adrian Alder, Vajiheh Safavi-Rizi, Javier Avalos, Klaus L. Kerres, Bjoern Scheler, Ralf Reski, Jari P. T. Valkonen, Salim Al-Babili, Aparna Balakrishna, Gertrud Wiedemann |
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| Přispěvatelé: | Universidad de Sevilla. Departamento de Genética |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Physiology Mutant Germination Plant Science Physcomitrella patens 01 natural sciences Carotenoid cleavage dioxygenase Conserved sequence Dioxygenases Phosphates 03 medical and health sciences chemistry.chemical_compound Gene Knockout Techniques Lactones Biosynthesis Arabidopsis Botany Phosphate starvation Chromatography High Pressure Liquid Disease Resistance Plant Diseases Plant Proteins 2. Zero hunger Strigolactones biology ATP synthase Carotenoid oxygenase Stereoisomerism 15. Life on land biology.organism_classification Biological Evolution Carlactone Carotenoids Bryopsida Orobanche ramosa 030104 developmental biology Biochemistry chemistry Sclerotinia sclerotiorum Mutation biology.protein Disease Susceptibility Heterocyclic Compounds 3-Ring 010606 plant biology & botany |
| Zdroj: | idUS. Depósito de Investigación de la Universidad de Sevilla instname |
| Popis: | In seed plants, strigolactones (SLs) regulate architecture and induce mycorrhizal symbiosis in response to environmental cues. SLs are formed by combined activity of the carotenoid cleavage dioxygenases (CCDs) 7 and 8 from 9-cis-β-carotene, leading to carlactone that is converted by cytochromes P450 (clade 711; MAX1 in Arabidopsis) into various SLs. As Physcomitrella patens possesses CCD7 and CCD8 homologs but lacks MAX1, we investigated if PpCCD7 together with PpCCD8 form carlactone and how deletion of these enzymes influences growth and interactions with the environment. We investigated the enzymatic activity of PpCCD7 and PpCCD8 in vitro, identified the formed products by high performance liquid chromatography (HPLC) and LC-MS, and generated and analysed ΔCCD7 and ΔCCD8 mutants. We defined enzymatic activity of PpCCD7 as a stereospecific 9-cis-CCD and PpCCD8 as a carlactone synthase. ΔCCD7 and ΔCCD8 lines showed enhanced caulonema growth, which was revertible by adding the SL analogue GR24 or carlactone. Wild-type (WT) exudates induced seed germination in Orobanche ramosa. This activity was increased upon phosphate starvation and abolished in exudates of both mutants. Furthermore, both mutants showed increased susceptibility to phytopathogenic fungi. Our study reveals the deep evolutionary conservation of SL biosynthesis, SL function, and its regulation by biotic and abiotic cues. Deutsche Forschungsgemeinschaft AL892/1-4 Academy of Finland 1253126 |
| Databáze: | OpenAIRE |
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