Evaluation of latex agglutination for detecting chlamydial antibody activity in psittacine bird sera by comparison with direct complement fixation
| Autor: | Francisco Arizmendi, James E. Grimes |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
0301 basic medicine
040301 veterinary sciences 030106 microbiology Sensitivity and Specificity Psittaciformes Serology 0403 veterinary science 03 medical and health sciences medicine Animals Serologic Tests Chlamydia psittaci Chlamydia General Veterinary biology Bird Diseases Complement Fixation Tests 04 agricultural and veterinary sciences Psittacosis medicine.disease Complement fixation test biology.organism_classification Antibodies Bacterial Latex fixation test Agglutination (biology) Titer Evaluation Studies as Topic Immunology biology.protein Antibody Latex Fixation Tests |
| Zdroj: | Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc. 5(2) |
| ISSN: | 1040-6387 |
| Popis: | Chlamydiosis in cage, domestic, and wild birds is caused by a gram-negative prokaryote, Chlamydia psittaci, which is an obligate intracellular parasite of a wide variety of host cells., Presentation of the disease can be subacute, acute, chronic, or the infection can be completely asymptomatic in mature avian hosts. Respiratory, digestive, urogenital tract, eye, and widespread systemic infections can occur. Because of the variability of clinical manifestations, it is difficult to diagnose the disease by simple clinical observation. Therefore, the use of laboratory tests to confirm or rule out chlamydiosis in live or dead birds is frequently necessary. 1,2,6,13,17,18,21 Different serologic tests have been used for detecting antibody activity induced by C. psittaci. One of the most commonly used tests has been the direct complement fixation (DCF). However, the serologic response of psittacine birds to C. psittaci is highly variable within and between species . 12,14 Nevertheless, a bird with a DCF titer ≥ 64 usually is considered to be currently infected. For rapid detection of Chlamydial antibody activity in psittacine birds’ serum, a latex agglutination (LA) has been described. Latex agglutination is a sensitive method that detected early Chlamydial antibody production in experimentally inoculated African grey parrots. The present study was undertaken to further evaluate the reliability of LA in the serodiagnosis of psittacine bird chlamydiosis. This was done by comparing LA and DCF test results. Two hundred fourteen sera were selected and divided into 3 groups for this study. Group I consisted of 82 sera from birds with various clinical signs such as lethargy, anorexia, sneezing, weight loss, conjunctivitis, diarrhea, yellow urate, hepatomegaly, splenomegaly, leucocytosis, and elevated SGOT enzyme. Group II consisted of 102 sera from birds with an unknown health status. Group III consisted of 30 sera from birds with a history of exposure to chlamydiainfected birds. Sera were from 5 different types of psittacine birds. In order to determine if there were any differences between titers in birds with early or chronic signs, Group I was further divided into 3 subgroups and results were tabulated accordingly: subgroup A birds were ill for 2-7 days; subgroup B birds were ill for 10-20 days; and subgroup C birds were ill for 21 days or longer. Antigen for DCF was made in cell culture and the test indicative of current infection with Chlamydia. Titers |
| Databáze: | OpenAIRE |
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