Impaired Control of Effector T Cells by Regulatory T Cells: A Clue to Loss of Oral Tolerance and Autoimmunity in Celiac Disease?
| Autor: | Nadia Kourda, Melika Ben Ahmed, Bertrand Meresse, Hechmi Louzir, Nadia Belhadj Hmida, Maha Abdeladhim, Majd Ben Rejeb, Yosra Said, Nadine Cerf-Bensussan, Amel Moussa |
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| Přispěvatelé: | Laboratoire d'immunologie clinique [Institut Pasteur de Tunis], Institut Pasteur de Tunis, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Département de Gastroentérologie et hépatologie, Hôpital Charles Nicolle [Rouen], Université de Tunis El Manar (UTM), Département de Pathologie, Interactions de l'épithelium intestinal et du système immunitaire (UMR_S 793), Institut National de la Recherche Agronomique (INRA)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), INSERM UMR 989 is supported by grants from INSERM, Fondation Princesse Grace, Association pour la Recherche Contre le Cancer, Ligue Contre le Cancer, Agence Nationale pour La Recherche, Fondation pour la Recherche Médicale-Equipes 2007 and Association Française des Intolérants au Gluten. N. Belhadj Hmida was supported by a grant from Le Réseau International des Instituts Pasteur., Université Tunis El Manar (UTM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Recherche Agronomique (INRA), Département d'Immunology clinique, Université Tunis El Manar ( UTM ) -Institut Pasteur de Tunis-Réseau International des Instituts Pasteur ( RIIP ), Université Tunis El Manar, Université Tunis El Manar ( UTM ), Interactions de l'épithelium intestinal et du système immunitaire ( UMR_S 793 ), Institut National de la Recherche Agronomique ( INRA ) -Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Pasteur Tunis, Institut, CHU Rouen, Normandie Université (NU)-Normandie Université (NU)-CHU Rouen, Normandie Université (NU)-Normandie Université (NU) |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
CD4-Positive T-Lymphocytes
Male Biopsy Autoimmunity Disease medicine.disease_cause T-Lymphocytes Regulatory Immune tolerance 0302 clinical medicine Celiac disease Intestinal Mucosa Interleukin-15 0303 health sciences medicine.diagnostic_test Reverse Transcriptase Polymerase Chain Reaction Effector Gastroenterology Forkhead Transcription Factors hemic and immune systems Regulatory T cells Middle Aged Flow Cytometry 3. Good health IL-15 Interleukin 15 Female [ SDV.MHEP.HEG ] Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology Adult Duodenum chemical and pharmacologic phenomena Statistics Nonparametric Flow cytometry Interferon-gamma 03 medical and health sciences Immune Tolerance medicine Humans 030304 developmental biology Hepatology business.industry Interleukin-2 Receptor alpha Subunit nutritional and metabolic diseases [SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology digestive system diseases [SDV.MHEP.HEG] Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology Impaired control Case-Control Studies Immunology business 030215 immunology |
| Zdroj: | American Journal of Gastroenterology American Journal of Gastroenterology, Nature Publishing Group: Open Access Hybrid Model Option A, 2011, epub ahead of print. ⟨10.1038/ajg.2011.397⟩ American Journal of Gastroenterology, Nature Publishing Group: Open Access Hybrid Model Option A, 2011, epub ahead of print. 〈10.1038/ajg.2011.397〉 The American Journal of Gastroenterology The American Journal of Gastroenterology, 2011, epub ahead of print. ⟨10.1038/ajg.2011.397⟩ |
| ISSN: | 0002-9270 1572-0241 |
| Popis: | International audience; OBJECTIVES:Regulatory T cells (Tregs) are instrumental for tolerance to self-antigens and dietary proteins. We have previously shown that interleukin (IL)-15, a cytokine overexpressed in the intestine of patients with celiac disease (CD), does not impair the generation of functional Tregs but renders human T cells resistant to Treg suppression. Treg numbers and responses of intestinal and peripheral T lymphocytes to suppression by Tregs were therefore compared in CD patients and controls.METHODS:Intraepithelial lymphocytes (IELs) and lamina propria lymphocytes (LPLs) were isolated from duodenal biopsy specimens of CD patients and controls. Concomitantly, CD4+CD25+ T lymphocytes (Tregs) were purified from blood. Responses of IELs and of LPLs, and peripheral lymphocytes (PBLs) to suppression by Tregs were tested by analyzing anti-CD3-induced proliferation and interferon (IFN)-γ production in the presence or absence of peripheral Tregs. Lamina propria and peripheral CD4+CD25+FOXP3+ T cells were assessed by flow cytometry.RESULTS:Although percentages of CD4+CD25+FOXP3+ LPLs were significantly increased in patients with active CD, proliferation and IFN-γ production of intestinal T lymphocytes were significantly less inhibited by autologous or heterologous Tregs in CD patients than in controls (P |
| Databáze: | OpenAIRE |
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