Using fluorescence polarization immunoassay for determination of erythrocyte methotrexate polyglutamates, a quick and easy test?
| Autor: | Birgit C. P. Koch, Robert de Jonge, Ethan den Boer, Ruud Huisman |
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| Přispěvatelé: | Laboratory Medicine, AII - Infectious diseases, Amsterdam Gastroenterology Endocrinology Metabolism, Cardiothoracic Surgery, Pharmacy, Erasmus MC other, Clinical Chemistry |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Pharmacology
musculoskeletal diseases Chromatography Erythrocytes Time Factors medicine.diagnostic_test Chemistry Coefficient of variation Chemistry Pharmaceutical Confidence interval Methotrexate Linear range Polyglutamic Acid Liquid chromatography–mass spectrometry Immunoassay Fluorescence Polarization Immunoassay medicine Humans Pharmacology (medical) Sample preparation Single-Blind Method Fluorescence anisotropy medicine.drug |
| Zdroj: | Therapeutic Drug Monitoring, 36(6), 819-823. Lippincott Williams and Wilkins Therapeutic Drug Monitoring, 36(6), 819-823. Lippincott Williams & Wilkins Den Boer, E, Koch, B C, Huisman, R & De Jonge, R 2014, ' Using fluorescence polarization immunoassay for determination of erythrocyte methotrexate polyglutamates, a quick and easy test? ', Therapeutic Drug Monitoring, vol. 36, no. 6, pp. 819-823 . https://doi.org/10.1097/FTD.0000000000000085 |
| ISSN: | 0163-4356 |
| Popis: | BACKGROUND:: The folate antagonist methotrexate (MTX) is the anchor drug in the treatment of rheumatoid arthritis. The therapeutic effects of MTX are attributed to the intracellular levels of MTX, present in the cell as polyglutamates (MTX-PGs). We aimed to validate an immunoassay for the measurement of MTX-PG in erythrocytes. METHODS:: Samples were analyzed by an adapted fluorescence polarization immune assay (FPIA) method on the FLx analyzer (Abbott). Cross-reactivity was determined in both plasma and erythrocyte pellet. In erythrocyte pellet, the imprecision, linearity, and lower limit of quantitation were determined. The method was compared with our in-house liquid chromatography tandem mass spectrometry (LC-MS/MS) method for total MTX-PG. RESULTS:: For the adapted FPIA method, a linear range of 25-1000 nmol/L (R = 0.993) was obtained for total MTX-PG in erythrocytes. A coefficient of variation of |
| Databáze: | OpenAIRE |
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