NonA and CPX Link the Circadian Clockwork to Locomotor Activity in Drosophila
| Autor: | Aoife McMahon, Hua Jin, Madelen M. Díaz, Michael Rosbash, Eranthie Weerapana, Allegra Fieldsend, Shalise M. Couvertier, Fang Guo, Weifei Luo |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Period (gene) Repressor Nerve Tissue Proteins Biology Article Animals Genetically Modified 03 medical and health sciences Complexin Downregulation and upregulation RNA interference Circadian Clocks Gene expression Animals Drosophila Proteins Circadian rhythm Transcription factor General Neuroscience Nuclear Proteins Circadian Rhythm Cell biology Adaptor Proteins Vesicular Transport 030104 developmental biology Drosophila Locomotion |
| Zdroj: | Neuron. 99:768-780.e3 |
| ISSN: | 0896-6273 |
| Popis: | Drosophila NonA and its mammalian ortholog NONO are members of the Drosophila behavior and human splicing (DBHS) family. NONO also has a strong circadian connection: it associates with the circadian repressor protein PERIOD (PER) and contributes to circadian timekeeping. Here, we investigate NonA, which is required for proper levels of evening locomotor activity as well as a normal free-running period in Drosophila. NonA is associated with the positive transcription factor CLOCK/CYCLE (CLK/CYC), interacts directly with complexin (cpx) pre-mRNA, and upregulates gene expression, including the gene cpx. Downregulation of cpx expression in circadian neurons phenocopies NonA downregulation, whereas cpx overexpression rescues the nonA RNAi phenotypes, indicating that cpx is an important NonA target gene. As the cpx protein contributes to proper neurotransmitter and neuropeptide release in response to calcium, these results and others indicate that this control is important for the normal circadian regulation of locomotor activity. |
| Databáze: | OpenAIRE |
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