Characterization of the Erwinia chrysanthemi gan locus involved in galactan catabolism
| Autor: | Anne-France Prouvost, Aurélie Delangle, Jean-Pierre Bohin, Virginie Cogez, Jean-Marie Lacroix, Nicole Hugouvieux Cotte-Pattat |
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| Přispěvatelé: | Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Microbiologie, adaptation et pathogénie (MAP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Université de Lille-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Sucrose
Mutant Pectobacterium chrysanthemi ATP-binding cassette transporter Biology Microbiology Galactans Chicory 03 medical and health sciences chemistry.chemical_compound Plant Microbiology Bacterial Proteins Molecular Biology 030304 developmental biology Solanum tuberosum 0303 health sciences 030306 microbiology Catabolism Dickeya chrysanthemi Temperature Periplasmic space Gene Expression Regulation Bacterial Galactan Glucose [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology chemistry Biochemistry Genes Bacterial Galactose Multigene Family Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Porin Mutation bacteria ATP-Binding Cassette Transporters Electrophoresis Polyacrylamide Gel Protons Genome Bacterial |
| Zdroj: | Journal of Bacteriology Journal of Bacteriology, American Society for Microbiology, 2007, 189 (19), pp.7053-7061. ⟨10.1128/JB.00845-07⟩ Journal of Bacteriology, 2007, 189 (19), pp.7053-7061. ⟨10.1128/JB.00845-07⟩ |
| ISSN: | 0021-9193 1098-5530 |
| Popis: | β-1,4-Galactan is a major component of the ramified regions of pectin. Analysis of the genome of the plant pathogenic bacteria Erwinia chrysanthemi revealed the presence of a cluster of eight genes encoding proteins potentially involved in galactan utilization. The predicted transport system would comprise a specific porin GanL and an ABC transporter made of four proteins, GanFGK 2 . Degradation of galactans would be catalyzed by the periplasmic 1,4-β-endogalactanase GanA, which released oligogalactans from trimer to hexamer. After their transport through the inner membrane, oligogalactans would be degraded into galactose by the cytoplasmic 1,4-β-exogalactanase GanB. Mutants affected for the porin or endogalactanase were unable to grow on galactans, but they grew on galactose and on a mixture of galactotriose, galactotetraose, galactopentaose, and galactohexaose. Mutants affected for the periplasmic galactan binding protein, the transporter ATPase, or the exogalactanase were only able to grow on galactose. Thus, the phenotypes of these mutants confirmed the functionality of the gan locus in transport and catabolism of galactans. These mutations did not affect the virulence of E. chrysanthemi on chicory leaves, potato tubers, or Saintpaulia ionantha , suggesting an accessory role of galactan utilization in the bacterial pathogeny. |
| Databáze: | OpenAIRE |
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