Optimum Conditions and LC-ESI-MS Analysis of Phenolic Rich Extract from Eucalyptus marginata L. under Maceration and Ultrasound-Assisted Extraction Methods Using Response Surface Methodology
| Autor: | Amir Bouallegue, Hanene Ghazghazi, Moufida A. Oueslati, Ridha Ben Salem, Hajer Riguene, Ghayth Rigane, Soumaya Hasni, Olfa Khedher |
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| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
Chromatography Article Subject biology Nutrition. Foods and food supply Extraction (chemistry) Flavonoid Hyperoside biology.organism_classification Protocatechuic acid chemistry.chemical_compound chemistry Maceration (wine) TX341-641 Gallic acid Response surface methodology Eucalyptus marginata Safety Risk Reliability and Quality Food Science |
| Zdroj: | Journal of Food Quality, Vol 2021 (2021) |
| ISSN: | 1745-4557 0146-9428 |
| DOI: | 10.1155/2021/5591022 |
| Popis: | Eucalyptus marginata L. has a significant value in traditional medicine and recently has been shown to possess many pharmacological properties in vitro. The main goal of the present study was to optimize the extraction parameters of phenolic compounds from Eucalyptus marginata L. leaves using the extraction technique assisted by ultrasound in comparison with maceration using response surface methodology as a predicted tool. Therefore, total phenolic and flavonoid contents have been optimized, taking into account four variables: extraction time, temperature, liquid-to-solid ratio, and ethanol concentration. The optimum ultrasound-assisted extraction method for total phenolic and total flavonoid contents was obtained by ensuring the following parameters: t = 49.9 min, T = 74.9°C, liquid-to-solid ratio = 39.5 ml/g, and ethanol = 58.48%. The optimum extract has been subjected to LC-ESI-MS analysis. This technique allowed us to identify ten phenolic compounds: four phenolic acids mainly gallic acid (27.77 ± 0.06 µg/g DW) and protocatechuic acid (37.66 ± 0.04 µg/g DW) and six flavonoid compounds such as quercetrin (150.78 ± 0.02 µg/g DW) and hyperoside (39.19 ± 0.03 µg/g DW). These green and efficient procedures should be a promising option to guide industrial design for the production of phenolic-rich plant extracts. |
| Databáze: | OpenAIRE |
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