The root‐knot nematode effector MiEFF18 interacts with the plant core spliceosomal protein SmD1 required for giant cell formation

Autor: Hervé Vaucheret, Bruno Favery, Jérémie Bazin, Michaël Quentin, Nhat-My Truong, Nathalie Marteu, Nathalie Bouteiller, Joffrey Mejias, Yongpan Chen, Shinichiro Sawa, Pierre Abad, Martin Crespi
Přispěvatelé: Institut Sophia Agrobiotech (ISA), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Côte d'Azur (UCA), Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Kumamoto University, China Agricultural University (CAU), Institut Jean-Pierre Bourgin (IJPB), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), INRA SPE department, INRA-Syngenta Targetome project, French-Japanese bilateral collaboration programme PHC SAKURA 2016 35891VD, French-Chinese bilateral collaboration program PHC XU GUANGQI 2020 45478PF, French Ministere de lEnseignement Superieur, de la Recherche et de lInnovation (MENRT grant), USTH fellowship, as part of the 911-USTH programme of the Ministry of Education and Training of The Socialist Republic of Vietnam, China Scholarship Council 201806350108, French-Japanese bilateral collaboration programme PHC SAKURA 2019 43006VJ, ANR-11-LABX-0028,SIGNALIFE,Réseau d'Innovation sur les Voies de Signalisation en Sciences de la Vie(2011), ANR-16-CE12-0032,SPLISIL,Dialogue épissage et extinction de l'ARN durant l'expression génétique(2016), ANR-10-LABX-0040,SPS,Saclay Plant Sciences(2010), Université Nice Sophia Antipolis (1965 - 2019) (UNS), Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: New Phytologist
New Phytologist, Wiley, 2021, 229 (6), pp.3408-3423. ⟨10.1111/nph.17089⟩
New Phytologist, 2021, 229 (6), pp.3408-3423. ⟨10.1111/nph.17089⟩
ISSN: 0028-646X
1469-8137
DOI: 10.1111/nph.17089⟩
Popis: International audience; The root-knot nematode Meloidogyne incognita secretes specific effectors (MiEFF) and induces the redifferentiation of plant root cells into enlarged multinucleate feeding 'giant cells' essential for nematode development. Immunolocalizations revealed the presence of the MiEFF18 protein in the salivary glands of M. incognita juveniles. In planta, MiEFF18 localizes to the nuclei of giant cells demonstrating its secretion during plant-nematode interactions. A yeast two-hybrid approach identified the nuclear ribonucleoprotein SmD1 as a MiEFF18 partner in tomato and Arabidopsis. SmD1 is an essential component of the spliceosome, a complex involved in pre-mRNA splicing and alternative splicing. RNA-seq analyses of Arabidopsis roots ectopically expressing MiEFF18 or partially impaired in SmD1 function (smd1b mutant) revealed the contribution of the effector and its target to alternative splicing and proteome diversity. The comparison with Arabidopsis galls data showed that MiEFF18 modifies the expression of genes important for giant cell ontogenesis, indicating that MiEFF18 modulates SmD1 functions to facilitate giant cell formation. Finally, Arabidopsis smd1b mutants exhibited less susceptibility to M. incognita infection, and the giant cells formed on these mutants displayed developmental defects, suggesting that SmD1 plays an important role in the formation of giant cells and is required for successful nematode infection.
Databáze: OpenAIRE
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