Immature Midbrain Dopaminergic Neurons Derived from Floor‐Plate Method Improve Cell Transplantation Therapy Efficacy for Parkinson's Disease
| Autor: | Shaoping Xie, Shunhui Wei, Mei-Chih Liao, Allen Chen, Shaul Reuveny, Steve Oh, Walter Hunziker, Li Zeng, Zhi Dong Zhou, Lifeng Qiu, Eng-King Tan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Pathology medicine.medical_specialty Parkinson's disease Neurodegeneration / Neurological Disorders Neurogenesis Floor‐plate Mice SCID Biology Cell therapy 03 medical and health sciences Mice Translational Research Articles and Reviews Neural Stem Cells Mesencephalon Mice Inbred NOD Tissue Engineering and Regenerative Medicine medicine Animals Humans Progenitor cell Embryonic Stem Cells Cells Cultured Floor plate Transplantation Parkinson's Disease Dopaminergic Neurons Dopaminergic Parkinson Disease Cell Biology General Medicine medicine.disease Embryonic stem cell Neural/Progenitor Stem Cells 030104 developmental biology Midbrain dopaminergic neurons Differentiation Cancer research Tyrosine hydroxylase Stem cell Developmental Biology Stem Cell Transplantation |
| Zdroj: | Stem Cells Translational Medicine |
| ISSN: | 2157-6580 2157-6564 |
| Popis: | Recent reports have indicated human embryonic stem cells-derived midbrain dopamine (mDA) neurons as proper cell resources for use in Parkinson's disease (PD) therapy. Nevertheless, no detailed and systematic study has been conducted to identify which differentiation stages of mDA cells are most suitable for transplantation in PD therapy. Here, we transplanted three types of mDA cells, DA progenitors (differentiated in vitro for 16 days [D16]), immature DA neurons (D25), and DA neurons (D35), into PD mice and found that all three types of cells showed high viability and strong neuronal differentiation in vivo. Both D25 and D35 cells showed neuronal maturation and differentiation toward TH+ cells and, accordingly, satisfactory behavioral functional recovery. However, transplanted D16 cells were less capable of producing functional recovery. These findings provide a valuable guideline for standardizing the differentiation stage of the transplantable cells used in clinical cell therapy for PD. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text