Activity of a dry mist-generated hydrogen peroxide disinfection system against methicillin-resistant Staphylococcus aureus and Acinetobacter baumannii

Autor: Canan Külah, Mehtap Yumusak, Nihal Pişkin, Zafer Mengeloglu, Güven Çelebi
Přispěvatelé: Zonguldak Bülent Ecevit Üniversitesi
Rok vydání: 2011
Předmět:
Zdroj: American Journal of Infection Control. 39:757-762
ISSN: 0196-6553
DOI: 10.1016/j.ajic.2010.12.003
Popis: Background: The aim of this study was to evaluate the activity of a dry mist-generated hydrogen peroxide (DMHP) system (Sterinis; Gloster Sante Europe, Labege cedex, France) against methicillin-resistant Staphylococcus aureus (MRSA) and Acinetobacter baumannii. Methods: McFarland 0.5 suspensions of 2 test bacteria, either pure or containing 5% sterile serum, were prepared and inoculated onto sterile stainless steel disks. Each disk in a Petri dish - with the Petri dish cover either closed or open - was placed in different locations in an intensive care unit room. Quantitative cultures were performed after the cycle. Results: No growth occurred on the disks in the absence of a barrier, except 1 disk containing serum. Existence of a barrier, as a drawer or a covered Petri dish, caused failure in the disinfection activity. The mean reduction in initial log 10 bacterial count was lower for both of the test bacteria in presence of a barrier: 4.44- to 4.70-log 10 colony-forming units (cfu) decrease was observed in absence of a barrier, whereas 1.49- to 3.79-log 10 cfu decrease was observed in presence of a barrier. When the culture results were compared according to organic load content, the mean (±standard deviation) reduction of initial contamination in pure and in serum containing MRSA suspensions was 4.25 ± 1.20- and 3.34 ± 1.89-log 10 cfu, respectively. The mean (±standard deviation) reduction in pure and in serum containing A baumannii suspensions was 4.34 ± 0.89- and 3.87 ± 1.26-log 10 cfu, respectively. The differences were statistically significant (P
Databáze: OpenAIRE
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