The cerium-induced liver injury and oxidative frug metabolism in DBA/2 and C57BL/6 mice
| Autor: | Frej Stenbäck, Holger Kraul, Penti Arvela, Olavi Pelkonen |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Male
C57BL/6 medicine.medical_specialty Ratón Toxicology Mixed Function Oxygenases Cytochrome P-450 CYP2A6 Mice Cytochrome P-450 Enzyme System Inbred strain Internal medicine Cytochrome P-450 CYP1A1 medicine Animals Liver injury biology Chemistry Cytochrome P450 Cerium Metabolism biology.organism_classification medicine.disease Immunohistochemistry Mice Inbred C57BL Endocrinology Liver Mice Inbred DBA Toxicity Microsomes Liver Microsome biology.protein Aryl Hydrocarbon Hydroxylases Oxidoreductases |
| Zdroj: | Toxicology. 69:1-9 |
| ISSN: | 0300-483X |
| DOI: | 10.1016/0300-483x(91)90148-t |
| Popis: | The influence of the known hepatotoxic agent, cerium (Ce) on the activity of liver microsomal monoxygenases, especially coumarin 7-hydroxylase (COH) was investigated in two inbred strains of male mice, DBA/2N and C57BL/6N. Ce was injected intravenously in three doses (0.5, 1.0 and 2.0 mg/kg body wt) and the animals were killed 24 or 72 h later. On the basis of histological assessment of the liver, C57BL/6N mice are apparently more resistant to the hepatotoxic effect of Ce. At 24 h, COH activity was increased in a dose-dependent manner in DBA/2 animals, whereas no change was seen in C57BL/6N animals. A significant increase in all other enzymes studied, cytochrome P-450 (P450), ethoxycoumarin O-deethylase and ethoxyresorufin O-deethylase, was seen in DBA/2 mice injected with the highest dose of Ce. At 72 h Ce increased CON activity, as well as other enzymes, in C57BL/6N mice in a dose-dependent manner, whereas in DBA/2 mice the increase was only seen after the two lower doses, the highest dose causing severe morphological changes in the liver structure and a clear decrease in COH and other activities. The distribution studies with Ce-141 showed that C57BL/6N livers contained more Ce than DBA/2 livers after the highest dose. |
| Databáze: | OpenAIRE |
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