| Popis: |
Numerous analytical methods are based on immune precipitation because the proper use of antibodies ensures a high degree of sensitivity and a remarkable specificity. In addition most methods based on immune precipitation have a fairy simple protocol. Immune precipitation has been known now for more than 85 years 1 and has been used in countless studies, yet its molecular mechanism is still incompletely understood. Here, fens Steensgaard describes a two-stage model of the process, in which the second step is not immune-specific. This has implications for attempts to reproduce conventional antisera with cocktails of monoclonal reagents. |
