Mapping 3-Dimensional Neovessel Organization Steps Using Micro-Computed Tomography in a Murine Model of Hindlimb Ischemia–Brief Report
| Autor: | Oses, Pierre, Renault, Marie-Ange, Chauvel, Rémi, Leroux, Lionel, Chevallereau, Pierre, Allières, Cécile, Daret, Danièle, Lamazière, Jean-Marie Daniel, Dufourcq, Pascale, Couffinhal, Thierry, Duplàa, Cécile |
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| Přispěvatelé: | Adaptation cardiovasculaire à l'ischemie, Université Bordeaux Segalen - Bordeaux 2-Institut National de la Santé et de la Recherche Médicale (INSERM), Supported by The European Vascular Genomics Network (grant #503254), the Fondation de France (grant #2006005678), Fondation pour la Recherche Médicale (DCV20070409258) and Communauté de Travail Pyrénnéenne (CTP). L.L. is the recipient of a grant from the Fondation pour la Recherche Médicale., European Project: 26841,EVGN, Boullé, Christelle, European Vascular Genomics Network - EVGN - 26841 - OLD |
| Rok vydání: | 2009 |
| Předmět: |
Neoprene
Pathology medicine.medical_specialty genetic structures Angiogenesis Ischemia Contrast Media Neovascularization Physiologic MESH: Imaging Three-Dimensional Hindlimb Mesenchymal Stem Cell Transplantation Article Neovascularization Mice MESH: Neoprene Imaging Three-Dimensional [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system MESH: Contrast Media medicine Animals MESH: Animals MESH: Mesenchymal Stem Cell Transplantation MESH: Mice Peripheral Vascular Diseases business.industry MESH: Hindlimb MESH: Barium Mesenchymal stem cell MESH: Peripheral Vascular Diseases Hindlimb ischemia Blood flow medicine.disease [SDV.MHEP.CSC] Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system Disease Models Animal Barium Arteriogenesis MESH: Disease Models Animal MESH: Ischemia medicine.symptom Tomography X-Ray Computed MESH: Tomography X-Ray Computed Cardiology and Cardiovascular Medicine business MESH: Neovascularization Physiologic |
| Zdroj: | Arteriosclerosis, Thrombosis, and Vascular Biology Arteriosclerosis, Thrombosis, and Vascular Biology, 2009, 29 (12), pp.2090-2. ⟨10.1161/ATVBAHA.109.192732⟩ |
| ISSN: | 1524-4636 1079-5642 |
| DOI: | 10.1161/atvbaha.109.192732 |
| Popis: | Objectives— Studying the mechanisms of neovascularization and evaluating the effects of proangiogenic strategies require accurate analysis of the neovascular network. We sought to evaluate the contribution of the microcomputed tomography (mCT) providing high-resolution 3-dimensional (3D) structural data, to a better comprehension of the well-studied mouse hindlimb postischemic neovascularization. Methods and Results— We showed a predominant arteriogenesis process in the thigh and a predominant angiogenesis-related process in the tibiofibular region, in response to ischemia during the first 15 days. After 15 days, mCT quantitative analysis reveals a remodeling of arterial neovessels and a regression depending on the restoration of the blood flow. We provided also new mCT data on the rapid and potent angiogenic effects of mesenchymal stem cell therapy on vessel formation and organization. We discussed the contribution of this technique compared with or in addition to data generated by the more conventional approaches. Conclusion— This study demonstrated that optimized mCT is a robust method for providing new insights into the 3D understanding of postischemic vessel formation. |
| Databáze: | OpenAIRE |
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