Determination of clozapine and its major metabolites in human plasma and red blood cells by high-performance liquid chromatography with ultraviolet absorbance detection
| Autor: | Jean-Marie Kinowski, Regis Aznar, Claire Guitton, Françoise Bressolle |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Erythrocytes
Chromatography medicine.diagnostic_test Metabolite Ethyl acetate General Chemistry Sensitivity and Specificity High-performance liquid chromatography chemistry.chemical_compound Column chromatography Drug Stability chemistry Therapeutic drug monitoring Liquid–liquid extraction Spectrophotometry medicine Humans Spectrophotometry Ultraviolet Clozapine Quantitative analysis (chemistry) Chromatography High Pressure Liquid Antipsychotic Agents |
| Zdroj: | Journal of Chromatography B: Biomedical Sciences and Applications. 690:211-222 |
| ISSN: | 0378-4347 |
| Popis: | An isocratic high-performance liquid chromatographic (HPLC) method with UV absorbance detection is described for the quantification of clozapine (8-chloro-11-(4'-methyl)piperazino-5H-dibenzo[b,e]-1,4-diazepine) and its two major metabolites in plasma and red blood cells (RBCs). The method involves sample clean-up by liquid-liquid extraction with ethyl acetate. The organic phase was back-extracted with 0.1 M hydrochloric acid. Loxapine served as the internal standard. The analytes were separated by HPLC on a Kromasil Ultrabase C18 analytical column (5 microns particle size; 250 x 4.6 mm I.D.) using acetonitrile-phosphate buffer pH 7.0 (48:52, v/v) as eluent and were measured by UV absorbance detection at 254 nm. The limits of quantiation were 20 ng/ml for clozapine and N-desmethylclozapine and 30 ng/ml for clozapine N-oxide. Recovery from plasma or RBCs proved to be higher than 62%. Precision, expressed as % C.V., was in the range 0.6-15%. Accuracy ranged from 96 to 105%. The method's ability to quantify clozapine and two major metabolites simultaneously with precision, accuracy and sensitivity makes it useful in therapeutic drug monitoring. |
| Databáze: | OpenAIRE |
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