Human Osteoblast Cell Behaviour on Titanium Discs Treated with Argon Plasma
| Autor: | María Rizo-Gorrita, Irene Luna-Oliva, Carolina González-Blanco, María-Ángeles Serrera-Figallo, Daniel Torres-Lagares, José-Luis Gutiérrez-Pérez |
|---|---|
| Přispěvatelé: | Universidad de Sevilla. Departamento de Estomatología |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Biocompatibility
surface decontamination Negative control chemistry.chemical_element 02 engineering and technology lcsh:Technology Article 03 medical and health sciences 0302 clinical medicine dental implants General Materials Science Viability assay Osteoblast cell titanium Cell adhesion lcsh:Microscopy lcsh:QC120-168.85 Titanium Osteoblasts Argon lcsh:QH201-278.5 osteoblasts/drug effects Chemistry lcsh:T Dental implants fungi Surface decontamination 030206 dentistry Plasma 021001 nanoscience & nanotechnology lcsh:TA1-2040 lcsh:Descriptive and experimental mechanics lcsh:Electrical engineering. Electronics. Nuclear engineering argon/therapeutic use 0210 nano-technology lcsh:Engineering (General). Civil engineering (General) lcsh:TK1-9971 Nuclear chemistry |
| Zdroj: | Materials Materials, Vol 12, Iss 11, p 1735 (2019) Volume 12 Issue 11 idUS. Depósito de Investigación de la Universidad de Sevilla instname |
| ISSN: | 1996-1944 |
| Popis: | (1) Background. Titanium is characterized by its biocompatibility and resistance to stress and fatigue. Treatment with argon plasma may favour growth of human osteoblasts with respect to cell adhesion and proliferation. The aim of this study was to analyse the behaviour of human osteoblasts (MG-63) on Grade IV and V titanium possessing a sand-blasted, acid-etched (SLA) surface. SLA is a widely used surface treatment to create micro- and macroretentions to enhance osteoconductive properties on the surface. (2) Methods. One group of each grade of titanium was decontaminated with argon plasma and compared. On each disc, 20 × 104 cells were cultivated for morphological analysis, study of cell viability (regarding a negative control [100% viability]) and mitochondrial energy balance. (3) Results. At 24 h titanium treated with SLA showed a higher percentage of cell viability (47.3 ± 8.1%) compared to titanium IV treated with argon plasma, which presented a percentage of 79.1 ± 1.1%. Grade V titanium treated with argon plasma presented a higher viability percentage 91.3 ± 3.0% whereas nontreated Grade V titanium presented 53.3 ± 4.0%. Cells cultivated on the surfaces with an argon-plasma treatment were enlarged in comparison to non-treated discs. The cells with smaller circularity with a greater spread and spindle shape were the ones cultivated on the Grade V titanium surface. Cells seeded on treated titanium IV and titanium V, treated or not, showed higher mitochondrial activity over nontreated titanium IV. (4) Conclusions. Cells cultivated on those Grade V titanium discs that were decontaminated with argon plasma presented higher levels of cell adhesion and proliferation, lower mitochondrial damage and a higher mean cell area compared to those not decontaminated with argon plasma. |
| Databáze: | OpenAIRE |
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