Anti-Oxidant, Anti-Hemolytic Effects of Crataegus aronia Leaves and Its Anti- Proliferative Effect Enhance Cisplatin Cytotoxicity in A549 Human Lung Cancer Cell Line
| Autor: | Firas Kobeissy, Islam Omairi, Salam Nasreddine |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
antiproliferative activity Lung Neoplasms antioxidant activity Antineoplastic Agents Apoptosis Pharmacology Hemolysis Antioxidants 03 medical and health sciences 0302 clinical medicine Cell Movement medicine Humans MTT assay Cytotoxicity Cell Proliferation A549 cell Cisplatin Crataegus Chemistry Plant Extracts Cell Cycle Drug Synergism General Medicine Cell cycle cells aggregation Cell aggregation Plant Leaves 030104 developmental biology A549 Cells 030220 oncology & carcinogenesis cells migration Aronia medicine.drug Research Article Crataegus aronia |
| Zdroj: | Asian Pacific Journal of Cancer Prevention : APJCP |
| ISSN: | 2476-762X |
| Popis: | Objective For Arabian traditional medicine, Crataegus aronia syn. Azarolus (L) Bosc. ex DC (Rosaceae) is widely used to treat diabetes, sexual weakness, cardiovascular diseases and cancer. The anti-cancerous and anti-hemolysis effects of the hydroalcoholic extract of this plant have never been investigated before. The present study aims to evaluate the biological activities of the hydroalcoholic extract of Crataegus aronia leaves in combination with cisplatin, one of the most widely employed chemotherapeutics, on A549 human lung cancer cell line. Methods The anti-oxidant and anti-proliferative activities of leaves, fruits, seeds of C. aronia were investigated by DPPH method and MTT assay; respectively. Cell migration activity was investigated by wound healing and by cell aggregation assays. The effect of C. aronia in inducing cell cycle arrest along with activating cell apoptosis was evaluated by flow cytometry and Western blot assays, respectively. Results Our results showed that C. aronia leaves (C. aronia L.) had the highest anti-oxidant and anti-proliferative activities. The leaves extract was potent against hemolysis of the human erythrocytes and showed elevated decrease in migration by reducing wound healing migration and by increasing cell aggregation. Finally, C. aronia L. treatment exhibited apoptotic activity on A549 cells by the down-regulation of PARP-1, caspase-3 and Bcl-2 proteins and by increasing the percentage of A549 cells in sub G0 cell cycle. Moreover, the co-treatment of C. aronia L. and cisplatin remarkably sensitised A549 cells to cisplatin. Conclusion The results suggested that C. aronia L. could be used as a potential treatment against human lung cancer exhibiting minimal side effects on human health. . |
| Databáze: | OpenAIRE |
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