Solute transport across the articular surface of injured cartilage
| Autor: | Hooi Chuan Chin, Mohammad Moeini, Thomas M. Quinn |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Cartilage
Articular Biophysics Contrast Media Sodium Iodide In Vitro Techniques Biochemistry Diffusion Extracellular matrix chemistry.chemical_compound medicine Animals Insulin Femur Chondroitin sulfate Fluorescein isothiocyanate Molecular Biology Fluorescent Dyes Cartilage Chondroitin Sulfates Biological Transport Dextrans Articular surface Molecular Weight Partition coefficient medicine.anatomical_structure Dextran chemistry Sodium iodide Cattle Fluorescein-5-isothiocyanate |
| Zdroj: | Archives of Biochemistry and Biophysics. 535:241-247 |
| ISSN: | 0003-9861 |
| DOI: | 10.1016/j.abb.2013.04.011 |
| Popis: | Solute transport through extracellular matrix (ECM) is important to physiology and contrast agent-based clinical imaging of articular cartilage. Mechanical injury is likely to have important effects on solute transport since it involves alteration of ECM structure. Therefore it is of interest to characterize effects of mechanical injury on solute transport in cartilage. Using cartilage explants injured by an established mechanical compression protocol, effective partition coefficients and diffusivities of solutes for transport across the articular surface were measured. A range of fluorescent solutes (fluorescein isothiocyanate, 4 and 40 kDa dextrans, insulin, and chondroitin sulfate) and an X-ray contrast agent (sodium iodide) were used. Mechanical injury was associated with a significant increase in effective diffusivity versus uninjured explants for all solutes studied. On the other hand, mechanical injury had no effects on effective partition coefficients for most solutes tested, except for 40 kDa dextran and chondroitin sulfate where small but significant changes in effective partition coefficient were observed in injured explants. Findings highlight enhanced diffusive transport across the articular surface of injured cartilage, which may have important implications for injury and repair situations. Results also support development of non-equilibrium methods for identification of focal cartilage lesions by contrast agent-based clinical imaging. |
| Databáze: | OpenAIRE |
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