Pegylated leptin antagonist with strong orexigenic activity in mice is not effective in chickens
| Autor: | A, Gertler, D, Shinder, S, Yosefi, M, Shpilman, C I, Rosenblum, M, Ruzal, E, Seroussi, M, Friedman-Einat, M, Freidman-Einat |
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| Rok vydání: | 2014 |
| Předmět: |
Leptin
Male medicine.medical_specialty animal structures Physiology media_common.quotation_subject Adipose tissue Biology Aquatic Science Feed conversion ratio Fats Eating Mice In vivo Orexigenic Internal medicine medicine Animals Humans Molecular Biology Ecology Evolution Behavior and Systematics media_common Leptin receptor Body Weight digestive oral and skin physiology Antagonist Appetite HEK293 Cells Endocrinology Insect Science Receptors Leptin Female Animal Science and Zoology Chickens hormones hormone substitutes and hormone antagonists Signal Transduction medicine.drug |
| Zdroj: | Journal of Experimental Biology. 217:2222-2222 |
| ISSN: | 1477-9145 0022-0949 |
| Popis: | Summary A chicken gene orthologous to human leptin receptor (LEPR) has been characterized and found to be active in leptin signaling in vitro in response to a variety of recombinant leptins and leptin-containing blood samples. However, the endogenous ligand of chicken LEPR (cLEPR) - the putative chicken leptin - has been reported by us and others to be undetectable at the DNA, mRNA, protein and activity levels. These reports have raised questions as to cLEPR's role. Here we analyzed the effects of a pegylated superactive mouse leptin antagonist (PEG-SMLA) in chicken. We showed that the leptin antagonist efficiently and specifically blocks leptin signaling through the cLEPR in vitro. The effect of the leptin antagonist was then studied in vivo by daily administration of 10 mg/kg for 10 consecutive days to White Leghorn female chickens (G. gallus), at the age of two weeks. Despite the efficient attenuation of the cLEPR in vitro, no effect was observed on body weight, feed intake, feed efficiency or fat accumulation in the treated birds. Since similar treatment in rodents leads to a highly pronounced increase in appetite and body weight that are observed from the first day of treatment, it is concluded that the cLEPR is not implicated in the control of appetite or adipose homeostasis in chickens. |
| Databáze: | OpenAIRE |
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