A Large-Scale Mutational Analysis of Two-Component Signaling Systems of Lonsdalea quercina Revealed that KdpD-KdpE Regulates Bacterial Virulence Against Host Poplar Trees
| Autor: | Chao-Ying Deng, Ruo-Lan Yang, Jin-Wei Wei, Aining Li, Wei Qian, Wei He |
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| Rok vydání: | 2018 |
| Předmět: |
DNA
Bacterial 0301 basic medicine Physiology Operon DNA Mutational Analysis Virulence Biology Genome Bacterial genetics 03 medical and health sciences Bacterial Proteins Gram-Negative Bacteria Gene Plant Diseases Palindromic sequence Genetics fungi Promoter Gene Expression Regulation Bacterial General Medicine Populus 030104 developmental biology Agronomy and Crop Science Signal Transduction |
| Zdroj: | Molecular Plant-Microbe Interactions®. 31:724-736 |
| ISSN: | 1943-7706 0894-0282 |
| DOI: | 10.1094/mpmi-10-17-0248-r |
| Popis: | Poplar, which is a dominant species in plant communities distributed in the northern hemisphere, is commonly used as a model plant in forestry studies. Poplar production can be inhibited by infections caused by bacteria, including Lonsdalea quercina subsp. populi, which is a gram-negative bacterium responsible for bark canker disease. However, the molecular basis of the pathogenesis remains uncharacterized. In this study, we annotated the two-component signal transduction systems (TCSs) encoded by the L. quercina subsp. populi N-5-1 genome and identified 18 putative histidine kinases and 24 response regulators. A large-scale mutational analysis revealed that 19 TCS genes regulated bacterial virulence against poplar trees. Additionally, the deletion of kdpE or overexpression of kdpD resulted in almost complete loss of bacterial virulence. We observed that kdpE and kdpD formed a bi-cistronic operon. KdpD exhibited autokinase activity and could bind to KdpE (Kd = 5.73 ± 0.64 μM). Furthermore, KdpE is an OmpR family response regulator. A chromatin immunoprecipitation sequencing analysis revealed that KdpE binds to an imperfect palindromic sequence within the promoters of 44 genes, including stress response genes Lqp0434, Lqp3037, and Lqp3270. A comprehensive analysis of TCS functions may help to characterize the regulation of poplar bark canker disease. |
| Databáze: | OpenAIRE |
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