Establishment and genetic characterization of cell lines derived from proliferating nasal polyps and sinonasal inverted papillomas
| Autor: | Surachat Chaiwiriyakul, Tipaya Ekalaksananan, Kanha Muisuk, Tohru Kiyono, Jureeporn Kampan, Pornthep Kasemsiri, Piti Ungarreevittaya, Watchareporn Teeramatwanich, Patravoot Vatanasapt, Thawaree Nukpook, Chamsai Pientong |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
Adolescent Science Mutant Nose Neoplasms Cell Culture Techniques Biology Article Cytokeratin Nasal Polyps Transduction Genetic Cell Line Tumor medicine Humans Cyclin D1 Cancer models Head and neck cancer Child Telomerase Cells Cultured Aged Cell Proliferation Papilloma Inverted Multidisciplinary Cell growth Chromosome Cyclin-Dependent Kinase 4 medicine.disease Molecular biology Primary tumor In vitro Cell culture Medicine Immortalised cell line Microsatellite Repeats |
| Zdroj: | Scientific Reports Scientific Reports, Vol 11, Iss 1, Pp 1-14 (2021) |
| ISSN: | 2045-2322 |
| Popis: | To better understand the pathogenesis of nasal polyps (NPs) and sinonasal inverted papillomas (SIPs), we aimed to establish cell lines from fresh tissues of NPs and SIPs and characterize them. Primary cell cultures were obtained from two NP tissues (NP2 and NP3) and one SIP tissue (IP4). All the cells were polygonal in shape, expressed cytokeratin 14, and had normal diploid chromosome status. HPV58 DNA was detected in NP3. To obtain immortal primary cells, NP2 and IP4 cells were transduced with a combination of mutant CDK4, cyclinD1 and TERT. These cells were thereafter named NP2/K4DT and IP4/K4DT, respectively. HPV58-positive NP3 cells were transduced with TERT alone, the resulting cells named NP3/T. Phenotypic and genotypic identity of original tissues and derived cells was investigated. All the cell cultures with transgenes were confirmed to be derived from their parental cells and primary tumor tissues by analysis of short tandem repeats (STR) and maintained in vitro growth, genetic profiles and gene expression characteristics of the primary cells. These virtually immortalized cells, as well as the primary cells, have potential as in vitro models for studying the pathogenesis of NPs and SIPs and for preclinical study to develop new therapeutic agents. |
| Databáze: | OpenAIRE |
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