Restricted Theiler's murine encephalomyelitis virus infection in murine macrophages induces apoptosis

Autor:	M L Jelachich, H L Lipton
Rok vydání:	1999
Předmět:	TUNEL assay biology Macrophages viruses Apoptosis hemic and immune systems DNA Fragmentation Virology Virus Enzyme Activation Mice Enzyme activator Theilovirus Cell culture Caspases Cardiovirus Infections In Situ Nick-End Labeling biology.protein Animals DNA fragmentation Caspase
Zdroj:	Journal of General Virology. 80:1701-1705
ISSN:	1465-2099 0022-1317
DOI:	10.1099/0022-1317-80-7-1701
Popis:	Increasing evidence suggests that macrophages (M(phi)s) are necessary for persistence of Theiler's murine encephalomyelitis virus (TMEV) in the mouse central nervous system. Analysis of BeAn virus infection in the Mphi cell lines P388D1, J774A.1 and PU5-1.8, which are intermediate in their state of differentiation and resemble multifunctional resident M(phi)s, revealed restricted TMEV growth. As a result of the restricted infection, these Mphi cell lines were induced to undergo apoptosis as demonstrated by cellular morphology, DNA fragmentation, caspase protease activity, and in individual cells, by terminal deoxytransferase dUTP nick-end labelling (TUNEL).
Databáze:	OpenAIRE
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