Attachment site mutants of bacteriophage lambda
| Autor: | Max Gottesman, Marc Shulman |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
Genetics
Microbial Genotype Mutant DNA Single-Stranded Coliphages DNA sequencing Chromosomes law.invention Bacteriophage Structural Biology law Transduction Genetic Molecular Biology Prophage Crosses Genetic Genetics Recombination Genetic biology Base Sequence Circular bacterial chromosome Chromosome Chromosome Mapping biology.organism_classification Molecular biology Models Structural Genes Mutation Recombinant DNA Recombination |
| Zdroj: | Journal of molecular biology. 81(4) |
| ISSN: | 0022-2836 |
| Popis: | Insertion and excision of the chromosome of phage λ occurs by recombination at special regions of the phage and bacterial chromosomes known as attachment sites (alt's) . We have isolated att mutants which display reduced recombination frequencies. The mutations are cis -dominant, trans -recessive, and can be crossed into a phage, bacterial or prophage att . These results suggest that the att's , although different over-all, include the same DNA sequence as part of their structure, and that the mutations reside in these sequences. Crosses between mutant and wild-type att's occasionally yield heterozygotes. This result suggests that recombination of the att's generates complementary single-strands via staggered nicks in these common sequences. Recombinant att's are then formed by the interannealing of single-strands of different att's followed by ligation. |
| Databáze: | OpenAIRE |
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