Sulfur-limitation-regulated proteins in Bacillus subtilis: a two-dimensional gel electrophoresis study
| Autor: | Valérie Labas, Valerie Vagner, Antoine Danchin, Agnieszka Sekowska, Evelyne Turlin, Sandrine Auger, Isabelle Martin-Verstraete, Jean-Pierre Le Caer, Jean-Yves Coppée |
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| Přispěvatelé: | Régulation de l'Expression Génétique (REG), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Neurobiologie et diversité cellulaire (NDC), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, This research was supported by LION BIOSCIENCE AG in the framework of a scientific collaboration, by grants from the Ministère de l’Education Nationale de la Recherche et de la Technologie, the Centre National de la Recherche Scientifique (URA 2171), the Institut Pasteur, the Université Paris 7 and the European Union Biotech Programme (contract ERBB104 CT960655)., We thank Luis A. Rivas, Marie-Françoise Hullo, Itaı̈ Yanai and Jerôme Chapuis for the construction of some of the mutants used in this study, Hans-Henrik Saxild for the gift of strain LCC1, Claire Mulot for her excellent technical assistance, Ivan Moszer for helpful discussions, and Philippe Bertin and Carmen Buchreiser for careful reading of the manuscript., Auger, Sandrine, Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris] |
| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: |
MESH: Sulfates / metabolism
Proteome Operon [SDV]Life Sciences [q-bio] Sulfur metabolism MESH: Recombinant Fusion Proteins / metabolism Bacillus subtilis MESH: Glutathione / metabolism Cysteine synthase Sulfur assimilation Electrophoresis Gel Two-Dimensional Gel electrophoresis 0303 health sciences biology Sulfates regulation MESH: Lac Operon Glutathione proteome analysis [SDV] Life Sciences [q-bio] [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Lac Operon Biochemistry MESH: Proteome MESH: Operon MESH: Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins MESH: Bacillus subtilis / metabolism chemistry.chemical_element Microbiology 03 medical and health sciences MESH: Gene Expression Profiling Bacterial Proteins MESH: Bacillus subtilis / genetics MESH: Bacterial Proteins / metabolism [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology 030304 developmental biology Two-dimensional gel electrophoresis Sulfur Compounds 030306 microbiology Gene Expression Profiling biology.organism_classification MESH: Electrophoresis Gel Two-Dimensional Molecular biology Sulfur chemistry MESH: Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization MESH: Bacterial Proteins / genetics Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization biology.protein sulfur metabolism MESH: Sulfur Compounds / metabolism |
| Zdroj: | Microbiology Microbiology, 2001, 147 (6), pp.1631-1640. ⟨10.1099/00221287-147-6-1631⟩ Microbiology, Microbiology Society, 2001, 147 (6), pp.1631-1640. ⟨10.1099/00221287-147-6-1631⟩ |
| ISSN: | 1350-0872 1465-2080 |
| Popis: | International audience; Little is known about the genes and enzymes involved in sulfur assimilation in Bacillus subtilis, or about the regulation of their expression or activity. To identify genes regulated by sulfur limitation, the authors used two- dimensional (2D) gel electrophoresis to compare the proteome of a wild-type strain grown with either sulfate or glutathione as sole sulfur source. A total of 15 proteins whose synthesis is modified under these two conditions were identified by matrix-assisted laser desorption/ionization time of flight (MALDI TOF) mass spectrometry. In the presence of sulfate, an increased amount of proteins involved in the metabolism of C1 units (SerA, GlyA, FolD) and in the biosynthesis of purines (PurQ, Xpt) and pyrimidines (Upp, PyrAA, PyrF) was observed. In the presence of glutathione, the synthesis of two uptake systems (DppE, SsuA), an oxygenase (SsuD), cysteine synthase (CysK) and two proteins of unknown function (YtmI, YurL) was increased. The changes in expression of the corresponding genes, in the presence of sulfate and glutathione, were monitored using slot-blot analyses and lacZ fusions. The ytmI gene is part of a locus of 12 genes which are co-regulated in response to sulfur availability. This putative operon is activated by a LysR-like regulator, YtlI. This is the first regulator involved in the control of expression in response to sulfur availability to be identified in B. subtilis. |
| Databáze: | OpenAIRE |
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