Astragalus membranaceus augment sperm parameters in male mice associated with cAMP-responsive element modulator and activator of CREM in testis
Autor: | Seong Kyu Park, Wonnam Kim, Do Rim Kim, Mun Seog Chang |
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Rok vydání: | 2015 |
Předmět: |
medicine.medical_specialty
CAMP-Responsive Element Modulator endocrine system Sperm count Short Communication lcsh:Medicine Semen analysis Biology Male infertility Andrology 03 medical and health sciences Semen quality 0302 clinical medicine Internal medicine medicine 030212 general & internal medicine education Sperm motility education.field_of_study cAMP response element modulator 030219 obstetrics & reproductive medicine Astragalus membranaceus (AM 黃芪 huáng qí) medicine.diagnostic_test urogenital system lcsh:R Activator of CREM in testis medicine.disease Epididymis Sperm Endocrinology medicine.anatomical_structure Complementary and alternative medicine Spermatogenesis |
Zdroj: | Journal of Traditional and Complementary Medicine Journal of Traditional and Complementary Medicine, Vol 6, Iss 3, Pp 294-298 (2016) |
ISSN: | 2225-4110 |
Popis: | Astragalus membranaceus BUNGE (AM; 黃芪 huáng qí) has been widely used as a medicinal herb for different kinds of diseases. AM treatment in vitro enhance sperm motility and ameliorates testicular toxicity, it has demonstrated the ability as a potential treatment for male infertility. In order to gain further insights on the molecular understanding of how AM enhances spermatogenesis, this study investigated whether AM has an affect on sperm parameters associated with cAMP response element modulator (CREM) and activator of CREM in testis (ACT) expression. Five-week-old male ICR mice were divided into four groups; control group and three different concentrations of AM treated groups. Each group was treated for 5 days a week for 5 weeks. Testis samples were collected for real time quantitative PCR and western blot analysis. Epididymis was taken out and used for sperm analysis using the computer assisted semen analysis (CASA) system. To facilitate expression of genes required for spermatogenesis, it is controlled by fine-tuning of CREM and its coactivator, ACT. AM treatment promotes CREM and ACT mRNA expression and also protein expression compared to control. AM enhances sperm values such as sperm count and motility compared to control. Overall, the study highlights, the ability of AM to increases CREM and ACT expression to facilitate sperm development and semen quality. Graphical abstract |
Databáze: | OpenAIRE |
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