BINDING OF NUCLEAR PROTEINS TO THE NEGATIVE REGULATORY ELEMENT OF THE IL-2 GENE IN LYMPHOCYTES FROM RHEUMATIC PATIENTS
| Autor: | Howard Dang, Norman Talal, Norma Vela-Roch, Eliezer Flescher |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Adult
Male T-Lymphocytes Immunology Negative regulatory element In Vitro Techniques Biology Lymphocyte Activation Biochemistry Arthritis Rheumatoid chemistry.chemical_compound medicine Humans Lupus Erythematosus Systemic Immunology and Allergy Electrophoretic mobility shift assay Nuclear protein Molecular Biology Gene Transcription factor Aged Binding Sites Binding protein Nuclear Proteins Hematology Middle Aged medicine.disease Molecular biology DNA-Binding Proteins chemistry Case-Control Studies Rheumatoid arthritis Interleukin-2 Female DNA |
| Zdroj: | Cytokine. 11:187-191 |
| ISSN: | 1043-4666 |
| DOI: | 10.1006/cyto.1998.0425 |
| Popis: | T lymphocytes from several autoimmune diseases including rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE) exhibit deficient mitogenic response in terms of proliferation and IL-2 production. The expression of the IL-2 gene is regulated by various transcription factors. One of these factors suppresses IL-2 expression and binds to the negative responsive element in the IL-2 gene 5′ flanking region (NRE-A). The authors hypothesized that the decreased production of IL-2 by T cells from RA and SLE patients is at least partially caused by high expression of the NRE-A binding protein. To test this hypothesis T cells from healthy donors and patients with RA and SLE were stimulated. Using the electrophoretic mobility shift assay we detected NRE-A DNA-binding proteins in the nuclei of the stimulated cells. No difference was found between NRE-A DNA binding in nuclear extracts of T cells taken from healthy donors and those taken from patients. The specificity of the DNA-protein interactions was ascertained through the use of unlabeled DNA competitors. No correlation was found between DNA-binding and the patients’ disease duration or medication. In conclusion, decreased IL-2 biosynthesis by T lymphocytes from RA and SLE patients can not be explained by abnormal expression of the NRE-A DNA-binding protein. |
| Databáze: | OpenAIRE |
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