19F NMR spectroscopy for the analysis of RNA secondary structure populations
| Autor: | Dagmar Graber, Ronald Micura, Holger Moroder |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Fluorine Radioisotopes
Magnetic Resonance Spectroscopy 19f nmr spectroscopy Ultraviolet Rays chemistry.chemical_element Nuclear magnetic resonance spectroscopy of nucleic acids Biochemistry Catalysis Protein Structure Secondary Nucleic acid secondary structure Colloid and Surface Chemistry Protein structure Nuclear magnetic resonance Nucleic Acids RNA Small Interfering Oligoribonucleotides Temperature RNA General Chemistry Hydrogen-Ion Concentration Non-coding RNA Combinatorial chemistry chemistry Models Chemical Fluorine Nucleic Acid Conformation Thermodynamics |
| Zdroj: | Journal of the American Chemical Society. 130(51) |
| ISSN: | 1520-5126 |
| Popis: | Labeling of RNA with site-specific fluorine atoms has become straightforward in recent years and in particular has become an integrated part of engineered functional RNA with therapeutic potential, e.g. for siRNA technologies. Here, we demonstrate that temperature-dependent one-dimensional (19)F NMR spectroscopy of oligoribonucleotides is a powerful tool to obtain direct information on the conformational behavior. The approach is particularly useful for the quantification of monomolecular vs bimolecular secondary structure equilibria. |
| Databáze: | OpenAIRE |
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