Experimental study of blood pressure and its impact on spontaneous hypertension in rats with Xin Mai Jia

Autor: Jian Xu, Huang Ning, Yun Jing, Jiajing Hu, Peng Li, Jierong Zhao, Yang Jing, Ya-Ling Yin, Zhang Mingxiang, Ping Song
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Male
0301 basic medicine
medicine.medical_specialty
Blood Pressure
Vasodilation
RM1-950
Rats
Inbred WKY
Vascular endothelial function
Muscle
Smooth
Vascular
Umbilical vein
Random Allocation
03 medical and health sciences
0302 clinical medicine
Rats
Inbred SHR
Internal medicine
medicine.artery
Renin–angiotensin system
Human Umbilical Vein Endothelial Cells
Animals
Humans
Medicine
Mesenteric arteries
Antihypertensive Agents
Cells
Cultured
Pharmacology
Aorta
Angiotensin II receptor type 1
Electrical impedance myography
business.industry
General Medicine
Rats
Primary hypertension
030104 developmental biology
Blood pressure
medicine.anatomical_structure
Endocrinology
030220 oncology & carcinogenesis
Spontaneously hypertensive rats
Renin-angiotensin-aldosterone system
Angiotensin II type 1 receptor
Hypertension
Xin Mai Jia
cardiovascular system
Endothelium
Vascular
Therapeutics. Pharmacology
business
Drugs
Chinese Herbal
Zdroj: Biomedicine & Pharmacotherapy, Vol 112, Iss, Pp-(2019)
ISSN: 0753-3322
Popis: Aim The aim of this study was to evaluate the antihypertensive effect of Xin Mai Jia (XMJ) and to explore the mechanism of its hypotensive effect. Methods A total of 50 spontaneously hypertensive rats (SHR) were randomised into five groups. A total of 30 Wistar-Kyoto rats were randomised into three groups, comprising the control group. All of the rats were administered medicine through a gastrogavage once a day for 8 weeks. The tail-cuff method was applied to their monitor blood pressure. After 8 weeks of treatment, serum NO, SOD activity, MDA level, ET, ALD, AngII, RE, and CGRP in the serum were detected in all of the rats. Pathological changes in the aorta were observed via haematoxylin-eosin (HE) and immunohistochemical staining. Vasodilation function was assessed by measuring acetylcholine-induced vessel relaxation in the rats’ organ chambers. The function of the mesenteric arteries was measured using DMT wire myography. Human aortic smooth muscle cells (HASMCs) and human umbilical vein endothelial cells (HUVECs) injury models were induced by hydrogen peroxide (H2O2). HASMCs and HUVECs were injured by H2O2 and then exposed to various drugs. HASMC and HUVEC migration was evaluated using the cell scratch test. The expression of the AT1 receptors (AT1R) in the HASMCs was detected via immunofluorescence (IFC) assay. Results After 8 weeks of treatment, XMJ reduced the systolic blood pressure of the SHR. XMJ significantly reduced the serum RE, AngII, ALD, and ET-1 levels and increased the content of CGRP and NO in the SHR, upregulated the SOD content, and downregulated MDA level of the SHR. XMJ improved pathological damage of the aorta to varying degrees, decreased the expression of AT1R in the SHR aortic vessels, and improved the mesenteric microvascular relaxation of the SHR. Cell experiments confirmed that XMJ inhibited the migration of the HUVECs and HASMCs induced by H2O2 and the expression of AT1R in the HASMCs. Conclusion XMJ had satisfactory hypotensive action on the SHR in this study. Its mechanism may be associated with inhibiting RAAS activity and improving RAAS function, inhibiting hypertensive-induced vascular diastolic dysfunction, and improving vascular endothelial function.
Databáze: OpenAIRE
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