HER2-intronic miR-4728-5p facilitates HER2 expression and accelerates cell proliferation and migration by targeting EBP1 in breast cancer

Autor: Chunyu Liu, Zhen Zhou, Yimin Quan, Xiangfeng Meng, Yuan Yuan, Chen-Yu Zhang, Yu Zhou, Mengchao Yu, Liuyi Li, Chen Wang, Xueliang Wang, Xiuting Hu, Minghui Liu, Xi Chen
Rok vydání: 2021
Předmět:
0301 basic medicine
Carcinogenesis
Receptor
ErbB-2
Kaplan-Meier Estimate
medicine.disease_cause
Biochemistry
0302 clinical medicine
Cell Movement
Breast Tumors
Medicine and Health Sciences
skin and connective tissue diseases
Multidisciplinary
Luciferase Assay
RNA-Binding Proteins
Transfection
Cancer Cell Migration
Enzymes
Nucleic acids
Cell Motility
Bioassays and Physiological Analysis
Oncology
Cell Processes
030220 oncology & carcinogenesis
MCF-7 Cells
Medicine
Cell lines
Biological cultures
Oxidoreductases
Luciferase
Research Article
Science
Down-Regulation
Breast Neoplasms
BT474 cells
Cell Migration
Biology
03 medical and health sciences
Breast cancer
Downregulation and upregulation
Cell Line
Tumor
Breast Cancer
microRNA
Genetics
medicine
Humans
Non-coding RNA
Adaptor Proteins
Signal Transducing
Cell Proliferation
Enzyme Assays
Natural antisense transcripts
Cell growth
Three prime untranslated region
Cancers and Neoplasms
Biology and Life Sciences
Proteins
Cancer
Cell Biology
medicine.disease
Introns
Gene regulation
Research and analysis methods
MicroRNAs
030104 developmental biology
Enzymology
Cancer research
RNA
Gene expression
Biochemical Analysis
Developmental Biology
Zdroj: PLoS ONE
PLoS ONE, Vol 16, Iss 2, p e0245832 (2021)
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0245832
Popis: HER2 amplification greatly contributes to the tumorigenesis of multiple cancers. Intronic miR-4728-5p is transcribed along with its host gene HER2. However, little is known about the role of miR-4728-5p in cancer. This study aims to elucidate the potential role of miR-4728-5p and the underlying mechanism in breast cancer. Kaplan-Meier analysis showed that higher expression of HER2 led to worse survival outcomes in breast cancer patients. The TCGA dataset revealed that compared to normal breast tissues, HER2 and miR-4728-5p levels were significantly upregulated in breast cancer tissues with a positive correlation. In functional assays, miR-4728-5p was confirmed to promote the proliferation and migration in breast cancer cell BT474. EBP1 was identified as a direct target of miR-4728-5p via bioinformatics and luciferase reporter assays. miR-4728-5p was further demonstrated to increase HER2 expression and promote cell proliferation and migration by directly inhibiting EBP1 in breast cancer. Taken together, the HER2-intronic miR-4728-5p/EBP1/HER2 feedback loop plays an important role in promoting breast cancer cell proliferation and migration. Our study provides novel insights for targeted therapies of breast cancer.
Databáze: OpenAIRE
Externí odkaz: