Activity of Experimental Mouthwashes and Gels Containing DNA-RNA and Bioactive Molecules against the Oxidative Stress of Oral Soft Tissues: The Importance of Formulations. A Bioreactor-Based Reconstituted Human Oral Epithelium Model
| Autor: | Eugenio Brambilla, Patrizia Procacci, Patrizia Sartori, Andrei C. Ionescu, Elena Vezzoli, Vincenzo Conte |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Mouthwashes
Pharmaceutical Science Organic chemistry 010501 environmental sciences medicine.disease_cause 030226 pharmacology & pharmacy 01 natural sciences Epithelium Article surfactants Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine QD241-441 Allantoin Pulmonary surfactant Drug Discovery Hyaluronic acid medicine biochemistry Humans oxidative stress Physical and Theoretical Chemistry Hydrogen peroxide Cytotoxicity Bisabolol 0105 earth and related environmental sciences bioactive compounds excipients Chemistry Mouth Mucosa mouthwash bioreactors DNA In vitro oral gels Biochemistry Chemistry (miscellaneous) Molecular Medicine RNA reconstituted oral epithelium Genetic Engineering Gels Oxidative stress |
| Zdroj: | Molecules Volume 26 Issue 10 Molecules, Vol 26, Iss 2976, p 2976 (2021) |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules26102976 |
| Popis: | Background: DNA-RNA compounds have shown promising protection against cell oxidative stress. This study aimed to assess the cytotoxicity, protective, or preventive effect of different experimental formulations on oral epithelia’s oxidative stress in vitro. Methods: Reconstituted human oral epithelia (RHOE) were grown air-lifted in a continuous-flow bioreactor. Mouthwashes and gels containing DNA-RNA compounds and other bioactive molecules were tested on a model of oxidative stress generated by hydrogen peroxide treatment. Epithelia viability was evaluated using a biochemical MTT-based assay and confocal microscopy structural and ultrastructural morphology was evaluated by light microscopy and TEM. Results: DNA-RNA showed non-cytotoxic activity and effectively protected against oxidative stress, but did not help in its prevention. Gel formulations did not express adequate activity compared to the mouthwashes. Excipients played a fundamental role in enhancing or even decreasing the bioactive molecules’ effect. Conclusion: A mouthwash formulation with hydrolyzed DNA-RNA effectively protected against oxidative stress without additional enhancement by other bioactive molecules. Active compounds, such as hyaluronic acid, β-Glucan, allantoin, bisabolol, ruscogenin, and essential oils, showed a protective effect against oxidative stress, which was not synergistic with the one of DNA-RNA. Incorporation of surfactant agents showed a reduced, yet significant, cytotoxic effect. |
| Databáze: | OpenAIRE |
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