Endogenous hydrogen sulfide improves vascular remodeling through PPARδ/SOCS3 signaling
| Autor: | Sheng Jin, Yuhong Chen, Danyang Tian, Hongmei Xue, Xu Teng, Lin Xiao, Yuming Wu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Agonist PPARδ medicine.medical_specialty Contraction (grammar) Vascular smooth muscle medicine.drug_class SOCS Suppressor of cytokine signaling Endogeny Sodium hydrosulfide MMP Matrix metallopeptidase VSMC Vascular smooth muscle cell Article GW501516 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine ECM Extracellular matrix Internal medicine medicine Vascular remodeling SOCS3 lcsh:Science (General) ComputingMethodologies_COMPUTERGRAPHICS lcsh:R5-920 Multidisciplinary Hydrogen sulfide PPG DL-propargylglycine STAT Signal transducers and activators of transcription medicine.disease 030104 developmental biology Endocrinology chemistry H2S Hydrogen sulfide PPARδ Peroxisome proliferator activated receptor delta 030220 oncology & carcinogenesis cardiovascular system Signal transduction lcsh:Medicine (General) CSE Cystathionine-γ-lyase lcsh:Q1-390 |
| Zdroj: | Journal of Advanced Research Journal of Advanced Research, Vol 27, Iss, Pp 115-125 (2021) |
| ISSN: | 2090-1232 |
| Popis: | Graphical abstract Highlight • H2S deficiency derived from CSE depletion contributes to the vascular remodeling and transformation of vascular smooth muscle cells. • PPARδ/SOCS3 signaling pathway is decreased in CSE/H2S deficiency-associated transformation of VSMCs. • Reduced PPARδ is responsible for decreased SOCS3 expression under condition of CSE depletion. • CSE/H2S preserves SOCS3 production through PPARδ and inhibits inflammatory molecules production. Introduction Mounting evidences demonstrated the deficiency of hydrogen sulfide (H2S) facilitated the progression of cardiovascular diseases. However, the exact effects of H2S on vascular remodeling are not consistent. Objectives This study aimed to investigate the beneficial role of endogenous H2S on vascular remodeling. Methods CSE inhibitor, DL-propargylglycine (PPG) was used to treat mice and vascular smooth muscle cells (VSMCs). Sodium hydrosulfide (NaHS) was given to provide hydrogen sulfide. Vascular tension, H&E staining, masson trichrome staining, western blot and CCK8 were used to determine the vascular remodeling, expressions of inflammatory molecules and proliferation of VSMCs. Results The deficiency of endogenous H2S generated vascular remodeling with aggravated active and passive contraction, thicken aortic walls, collagen deposition, increased phosphorylation of STAT3, decreased production of PPARδ and SOCS3 in aortas, which were reversed by NaHS. PPG inhibited expression of PPARδ and SOCS3, stimulated the phosphorylation of STAT3, increased inflammatory molecules production and proliferation rate of VSMCs which could all be corrected by NaHS supply. PPARδ agonist GW501516 offered protections similar to NaHS in PPG treated VSMCs. Aggravated active and passive contraction in PPG mice aortas, upregulated p-STAT3 and inflammatory molecules, downregulated SOCS3 and phenotype transformation in PPG treated VSMCs could be corrected by PPARδ agonist GW501516 treatment. On the contrary, PPARδ antagonist GSK0660 exhibited opposite effects on vascular contraction in aortas, expressions of p-STAT3 and SOCS3 in VSMCs compared with GW501516. Conclusion In a word, endogenous H2S protected against vascular remodeling through preserving PPARδ/SOCS3 anti-inflammatory signaling pathway. Deficiency of endogenous H2S should be considered as a risk factor for VSMCs dysfunction |
| Databáze: | OpenAIRE |
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