Inhibition of Niemann-Pick C1-like protein 1 by ezetimibe reduces uptake of deuterium-labeled vitamin D in mice
| Autor: | Anja C. Baur, Alexandra Schutkowski, Gabriele I. Stangl, Mikis Kiourtzidis, Julia Kühn, Frank Hirche |
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| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Vitamin medicine.medical_specialty Endocrinology Diabetes and Metabolism Clinical Biochemistry Absorption (skin) Hydroxylation Biochemistry Intestinal absorption Mixed Function Oxygenases Niemann-Pick C1-Like Protein 1 Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Endocrinology Ezetimibe Internal medicine medicine Vitamin D and neurology Animals Vitamin D Molecular Biology Calcifediol Chemistry Cholesterol Anticholesteremic Agents Membrane Transport Proteins Vitamins Cell Biology Deuterium Sterol Mice Inbred C57BL 030104 developmental biology Parathyroid Hormone 030220 oncology & carcinogenesis Molecular Medicine medicine.drug |
| Zdroj: | The Journal of Steroid Biochemistry and Molecular Biology. 197:105504 |
| ISSN: | 0960-0760 |
| DOI: | 10.1016/j.jsbmb.2019.105504 |
| Popis: | For a long time, orally ingested vitamin D was assumed to enter the body exclusively via simple passive diffusion. Recent data from in vitro experiments have described Niemann-Pick C1-like protein 1 (Npc1l1) as an important sterol transporter for vitamin D absorption. However, short-term applications of ezetimibe, which inhibits Npc1l1, were not associated with reduced vitamin D uptake in animals and humans. The current study aimed to elucidate the effect of long-term inhibition of Npc1l1 by ezetimibe on the uptake and storage of orally administered triple deuterated vitamin D3 (vitamin D3-d3). Therefore, 30 male wild-type mice were randomly assigned into three groups and received diets with 25 μg/kg of vitamin D3-d3 that contained 0 (control group), 50 or 100 mg/kg ezetimibe for six weeks. Mice fed diets with 50 or 100 mg/kg ezetimibe had lower circulating levels of cholesterol than control mice (-12 %, -15 %, P |
| Databáze: | OpenAIRE |
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