Rapid and sensitive detection of cardiac troponin I using a force enhanced immunoassay with nanoporous membrane
| Autor: | Ying Xiong, Peng Li, Yong Zhang, Hao Shang, Sandeep Kakade, Gil U. Lee, Won-Suk Chang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Analyte
Cardiac troponin Myocardial Infarction 02 engineering and technology 010402 general chemistry 01 natural sciences Nanopores Troponin I medicine Humans General Materials Science cardiovascular diseases Point of care Immunoassay medicine.diagnostic_test Chemistry 021001 nanoscience & nanotechnology 3. Good health 0104 chemical sciences Improved performance Membrane cardiovascular system Nanoporous membrane 0210 nano-technology Biomarkers Biomedical engineering |
| Zdroj: | Nanoscale. 12(23) |
| ISSN: | 2040-3372 |
| Popis: | There is a need for point of care diagnostic technologies that are rapid, sensitive, easy to use, and relatively inexpensive. In this article we describe an assay that uses an antibody functionalized nanoporous membrane and superparamagnetic beads to capture and detect human cardiac troponin I (cTnI), which is an important biomarker for acute myocardial infarction (AMI). The membrane assisted force differentiation assay (mFDA) is capable of detecting cTnI at a sensitivity of 0.1 pg ml-1 in 15% serum in less than 16 minutes, which is a significant improvement in performance over conventional lateral flow immuosorbant assays. The speed of this assay results from the rapid concentration of cTnI on the surface of the nanoporous membrane and the use of the magnetic beads to react with the analyte, which rapidly react with the immobilized cTnI. The increased sensitivity of assay results from the use of magnetically controlled forces that reduce the nonspecific background and modify both the on-rate and off-rate. We believe that the improved performance and ease of application of the mFDA will make it useful in the early identification of AMI as well as other diseases based on the detection of 1 pg ml-1 variations in the concentrations cTnI in blood. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|