Confronting FCP structure with ultrafast spectroscopy data: evidence for structural variations
| Autor: | Ramūnas Augulis, Claudia Büchel, Leonas Valkunas, Bruno Robert, Andrius Gelzinis |
|---|---|
| Přispěvatelé: | Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Laboratoire Bioénergétique Membranaire et Stress (LBMS), Département Biochimie, Biophysique et Biologie Structurale (B3S), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2021 |
| Předmět: |
Chlorophyll
Protein Conformation Energy transfer [SDV]Life Sciences [q-bio] Cyclotella meneghiniana Light-Harvesting Protein Complexes General Physics and Astronomy Trimer Xanthophylls 010402 general chemistry 01 natural sciences 03 medical and health sciences Protein structure 14. Life underwater Phaeodactylum tricornutum Amino Acid Sequence Physical and Theoretical Chemistry Spectroscopy 030304 developmental biology Diatoms 0303 health sciences biology Chemistry Chlorophyll A Chaetoceros gracilis biology.organism_classification 0104 chemical sciences Diatom Spectrometry Fluorescence Energy Transfer Chemical physics |
| Zdroj: | Physical Chemistry Chemical Physics Physical Chemistry Chemical Physics, 2020, ⟨10.1039/D0CP05578F⟩ |
| ISSN: | 1463-9084 1463-9076 |
| Popis: | Publisher: The Royal Society of Chemistry; Diatoms are a major group of algae, responsible for a quarter of the global primary production on our planet. Their adaptation to marine environments is ensured by their light-harvesting antenna – the fucoxanthin–chlorophyll protein (FCP) complex, which absorbs strongly in the blue-green spectral region. Although these essential proteins have been the subject of many studies, for a long time their comprehensive description was not possible in the absence of structural data. Last year, the 3D structures of several FCP complexes were revealed. The structure of an FCP dimer was resolved by crystallography for the pennate diatom Phaeodactylum tricornutum [W. Wang et al., Science, 2019, 363, 6427] and the structure of the PSII supercomplex from the centric diatom Chaetoceros gracilis, containing several FCPs, was obtained by electron microscopy [X. Pi et al., Science, 2019, 365, 6452; R. Nagao et al., Nat. Plants, 2019, 5, 890]. In this Perspective article, we evaluate how precisely these structures may account for previously published ultrafast spectroscopy results, describing the excitation energy transfer in the FCP from another centric diatom Cyclotella meneghiniana. Surprisingly, we find that the published FCP structures cannot explain several observations obtained from ultrafast spectroscopy. Using the available structures, and results from electron microscopy, we construct a trimer-based FCP model for Cyclotella meneghiniana, consistent with ultrafast experimental data. As a whole, our observations suggest that the structures from the proteins belonging to the FCP family display larger variations than the equivalent LHC proteins in plants, which may reflect species-specific adaptations or original strategies for adapting to rapidly changing marine environments. |
| Databáze: | OpenAIRE |
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