Detection of insulin mRNA in the peripheral blood after human islet transplantion predicts deterioration of metabolic control

Autor: Thierry Berney, Philippe Morel, A. M. James Shapiro, P.Y. Benhamou, M-C Brulhart, Domenico Bosco, Jacques Philippe, Christian Toso, Anne Wojtusciszyn, Mathieu Pierre Jean Armanet, Reto M. Baertschiger, Aline Mamin, Sandrine Demuylder-Mischler, Beate Ritz-Laser
Přispěvatelé: Centre de pharmacologie et innovation dans le diabète (CPID), Université Montpellier 1 (UM1)-Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2006
Předmět:
Male
RNA
Messenger/genetics
medicine.medical_treatment
Islets of Langerhans Transplantation
030230 surgery
0302 clinical medicine
Leukocytes
Immunology and Allergy
Insulin
Pharmacology (medical)
Whole blood
ddc:616
0303 health sciences
geography.geographical_feature_category
MESH: Middle Aged
ddc:617
Graft Survival
Insulin/genetics
[SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism
Middle Aged
Islet
Reverse transcription polymerase chain reaction
surgical procedures
operative
Leukocytes/metabolism
Female
Islets of Langerhans/pathology/physiology
Adult
medicine.medical_specialty
endocrine system
MESH: Graft Survival
MESH: Insulin
MESH: Leukocytes
03 medical and health sciences
Islets of Langerhans
Internal medicine
Diabetes mellitus
medicine
Humans
RNA
Messenger
Pancreatic hormone
030304 developmental biology
MESH: RNA
Messenger
Transplantation
geography
Type 1 diabetes
MESH: Humans
business.industry
MESH: Islets of Langerhans
MESH: Adult
medicine.disease
MESH: Male
Endocrinology
MESH: Islets of Langerhans Transplantation
business
MESH: Female
Zdroj: American Journal of Transplantation, Vol. 6, No 7 (2006) pp. 1704-11
BASE-Bielefeld Academic Search Engine
American Journal of Transplantation
American Journal of Transplantation, Wiley, 2006, 6 (7), pp.1704-11. ⟨10.1111/j.1600-6143.2006.01373.x⟩
ISSN: 1600-6135
1600-6143
DOI: 10.1111/j.1600-6143.2006.01373.x⟩
Popis: International audience; Recent updates of the Edmonton trial have shown that insulin independence is progressively lost in approximately 90% of islet transplant recipients over the first 5 years. Early prediction of islet graft injury could prompt the implementation of strategies attempting to salvage the transplanted islets. We hypothesize that islet damage is associated with the release and detection of insulin mRNA in the circulating blood. Whole blood samples were prospectively taken from 19 patients with type 1 diabetes receiving 31 islet transplants, immediately prior to transplantation and at regular time-points thereafter. After RNA extraction, levels of insulin mRNA were determined by quantitative reverse tran-scriptase-polymerase chain reaction. All patients exhibited a primary peak of insulin mRNA immediately after transplantation, without correlation of duration and amplitude with graft size or outcome. Twenty-five subsequent peaks were observed during the follow-up of 17 transplantations. Fourteen secondary peaks (56%) were closely followed by events related to islet graft function. Duration and amplitude of peaks were higher when they heralded occurrence of an adverse event. Peaks of insulin mRNA can be detected and are often associated with alterations of islet graft function. These data suggest that insulin mRNA detection in the peripheral blood is a promising method for the prediction of islet graft damage.
Databáze: OpenAIRE
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