A System for Continuous Pre- to Post-reperfusion Intra-carotid Cold Infusion for Selective Brain Hypothermia in Rodent StrokeModels
| Autor: | Jae H. Choi, Yi Wang, Sven Poli, Mohammed A. Almekhlafi, Ulf Ziemann |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Rodent model medicine.medical_specialty Neurology medicine.medical_treatment Ischemia Rodentia Blood volume Hypothermia 030204 cardiovascular system & hematology Neuroprotection Brain Ischemia Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Hypothermia Induced medicine.artery medicine Animals cardiovascular diseases Intra-arterial cold infusion Endovascular thrombectomy Saline Stroke Ischemic stroke business.industry General Neuroscience Brain Selective brain cooling medicine.disease Rats Anesthesia Reperfusion Original Article Neurology (clinical) Internal carotid artery medicine.symptom Cardiology and Cardiovascular Medicine business 030217 neurology & neurosurgery |
| Zdroj: | Translational Stroke Research |
| ISSN: | 1868-601X 1868-4483 |
| DOI: | 10.1007/s12975-020-00848-3 |
| Popis: | Intra-carotid cold infusion (ICCI) appears as a promising method for hypothermia-mediated brain protection from ischemic stroke. Recent clinical pilot studies indicate easy implementation of ICCI into endovascular acute ischemic stroke treatment. Current rodent ICCI-in-stroke models limit ICCI to the post-reperfusion phase. To establish a method for continuous ICCI over the duration of intra-ischemia to post-reperfusion in rodent stroke models, a novel system was developed. Eighteen male Sprague-Dawley rats were included. Intraluminal filament method was used for transient middle cerebral artery occlusion (MCAO). Normal saline (~ 0 °C) was delivered (≤ 2.0 mL/min) into the internal carotid artery via a customized infusion system without interruption during MCAO (intra-ischemia) to after filament withdrawal (post-reperfusion). Bilateral cortical and striatal temperatures were monitored. Hypothermia goals were a temperature reduction in the ischemic hemisphere by 2 °C prior to reperfusion and thereafter maintenance of regional brain hypothermia at ~ 32 °C limiting the administered ICCI volume to ½ of each rat’s total blood volume. During ischemia, maximum brain cooling rate was achieved with ICCI at 0.5 mL/min. It took 2 min to reduce ischemic striatal temperature by 2.3 ± 0.3 °C. After reperfusion, brain cooling was continued at 2 mL/min ICCI first (over 42 s) and maintained at 32.1 ± 0.3 °C at 0.7 mL/min ICCI over a duration of 15 ± 0.8 min. ICCI (total 12.6 ± 0.6 mL) was uninterrupted over the duration of the studied phases. First system that allows continuous ICCI during the phases of intra-ischemia to post-reperfusion in small animals for selective brain cooling and for investigations of other neuroprotective infusions. |
| Databáze: | OpenAIRE |
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