Snapshot imprinting: Rapid identification of cancer cell surface proteins and epitopes using molecularly imprinted polymers
| Autor: | Alan C. Spivey, Salvador Macip, Eric O. Aboagye, Stanislav S. Piletsky, Rajinder Singh, Donald J. L. Jones, Marta Braga, Elena V. Piletska, Sergey A. Piletsky, Thong Huy Cao, Marta Poblocka |
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| Přispěvatelé: | Imperial College Healthcare NHS Trust- BRC Funding, Medical Research Council |
| Rok vydání: | 2021 |
| Předmět: |
Proteomics
Technology Chemistry Multidisciplinary Cells EGFR Materials Science Biomedical Engineering Pharmaceutical Science Bioengineering Materials Science Multidisciplinary Computational biology Epitope Epitopes 0903 Biomedical Engineering NANOPARTICLES General Materials Science HEAD Imprinting (psychology) Nanoscience & Nanotechnology Cancer Science & Technology 1007 Nanotechnology Cluster of differentiation Chemistry Molecularly imprinted polymer Molecular diagnostics Transmembrane protein Drug development Molecularly imprinted polymers Physical Sciences Science & Technology - Other Topics SECRETION Molecular imprinting STEM-CELLS Biotechnology |
| Popis: | Proteomic mapping of cell surfaces is an invaluable tool for drug development and clinical diagnostics. This work describes a new ‘snapshot imprinting’ method designed to obtain proteomic maps of cell surfaces, with the aim of identifying cell surface markers and epitopes for diagnostic and therapeutic applications. The analysis of two cancer cell lines, HN5 and MDA-MB-468, is described herein as a proof of concept, along with the selective targeting of three identified epitopes of epidermal growth factor receptor using molecularly imprinted polymer nanoparticles. 438 proteins were identified using this technique, with 283 considered to be transmembrane or extracellular proteins. The major advantage of the molecular imprinting approach developed here is the ability to analyse cell surface proteins without tedious fractionation, affinity separation or labelling. We believe that this system of protein analysis may provide a basic molecular diagnostics toolbox for precise, personalised treatment of cancer and other diseases. |
| Databáze: | OpenAIRE |
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