Increased association of CD38 with lipid rafts in T cells from patients with systemic lupus erythematosus and in activated normal T cells
| Autor: | Mercedes Zubiaur, Norberto Ortego-Centeno, Enrique Raya-Alvarez, María-del-Carmen Navarro, Esther J. Pavón, José-Luis Callejas-Rubio, Francisco Navarro-Pelayo, Jaime Sancho, Pilar M. Muñoz |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
CD3 Complex
T cell Immunology CD4-CD8 Ratio Receptors Antigen T-Cell Lymphocyte Activation Polyethylene Glycols Interleukin 21 Membrane Microdomains T-Lymphocyte Subsets immune system diseases medicine Humans Lupus Erythematosus Systemic Plant Oils Cytotoxic T cell IL-2 receptor Antigen-presenting cell Molecular Biology CD40 biology ZAP70 Natural killer T cell ADP-ribosyl Cyclase 1 Cell biology medicine.anatomical_structure Solubility biology.protein Mitogens |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 0161-5890 |
| DOI: | 10.1016/j.molimm.2005.05.002 |
| Popis: | In this study we have determined whether there is a relationship between CD38 expression on T cells, its distribution in different membrane microdomains, and T cell activation in SLE patients. The data show that CD38 expression is augmented in ex vivo CD3+, CD4+, CD8+, and CD25+ SLE T cells, which correlates with its increased insolubility in Brij 98 detergent, and its translocation into lipid rafts. Moreover, SLE T cells show an altered CD4:CD8 ratio, which is due to a decreased proportion of CD4+ T cells and a concomitant increase in the proportion of CD8 + T cells. These data are consistent with the increased CD38 expression and lipid raft formation, and the significant reduction in the CD4:CD8 ratio observed in mitogen-stimulated normal T cells as compared with that in ex vivo untouched normal T cells. Increased expression of CD38 in floating rafts from SLE T cells, or from activated normal T cells may modulate TCR signaling by providing or sequestering signaling molecules to the engaged TCR. © 2005 Elsevier Ltd. All rights reserved. |
| Databáze: | OpenAIRE |
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