Sas3 and Ada2(Gcn5)-dependent histone H3 acetylation is required for transcription elongation at the de-repressed FLO1 gene
| Autor: | Michael Church, Mohamed M. Alhussain, Sari Pennings, Alastair B. Fleming, Kim C. Smith |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Saccharomyces cerevisiae Proteins Transcription Elongation Genetic Chromosomal Proteins Non-Histone genetic processes Saccharomyces cerevisiae SAP30 Biology Histones 03 medical and health sciences Histone H1 Cell Wall Gene Expression Regulation Fungal Histone H2A Histone methylation Genetics Histone code Nucleosome Histone H3 acetylation Promoter Regions Genetic Histone Acetyltransferases 030102 biochemistry & molecular biology Gene regulation Chromatin and Epigenetics Nuclear Proteins Acetylation HDAC4 Cell biology Nucleosomes Repressor Proteins enzymes and coenzymes (carbohydrates) 030104 developmental biology Mannose-Binding Lectins RNA Polymerase II Protein Binding Transcription Factors |
| Zdroj: | Nucleic Acids Research Church, M, Smith, K C, Mubarack, M M, Pennings, S & Fleming, A B 2017, ' Sas3 and Ada2(Gcn5)-dependent histone H3 acetylation is required for transcription elongation at the de-repressed FLO1 gene ', Nucleic Acids Research, vol. 45, no. 8 . https://doi.org/10.1093/nar/gkx028 |
| ISSN: | 1362-4962 |
| DOI: | 10.1093/nar/gkx028 |
| Popis: | The S. cerevisiae FLO1 gene encodes a cell wall protein that imparts cell-cell adhesion. FLO1transcription is regulated via the antagonistic activities of the Tup1-Cyc8 co-repressor and Swi-Snfco-activator complexes. Tup1-Cyc8 represses transcription through the organisation of stronglypositioned, hypoacetylated nucleosomes across gene promoters. Swi-Snf catalyses remodelling ofthese nucleosomes in a mechanism involving histone acetylation that is poorly understood. Here,we show that FLO1 de-repression is accompanied by Swi-Snf recruitment, promoter histone eviction,and Sas3 and Ada2(Gcn5)-dependent histone H3K14 acetylation. In the absence of H3K14acetylation, Swi-Snf recruitment and histone eviction proceed, but transcription is reduced,suggesting these processes, while essential, are not sufficient for de-repression. Further analysis inthe absence of H3K14 acetylation reveals RNAP II recruitment at the FLO1 promoter still occurs, butRNAP II is absent from the gene-coding region, demonstrating Sas3 and Ada2-dependent histone H3acetylation is required for transcription elongation. Analysis of the transcription kinetics at othergenes reveals shared mechanisms coupled to a distinct role for histone H3 acetylation, essential atFLO1, downstream of initiation. We propose histone H3 acetylation in the coding region providesrate-limiting control during the transition from initiation to elongation which dictates whether thegene is permissive for transcription. |
| Databáze: | OpenAIRE |
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