Stimulation of adrenergic development in neural crest cultures by a reconstituted basement membrane-like matrix is inhibited by agents that elevate cAMP

Autor: M. E. Forbes, Gerald D. Maxwell
Rok vydání: 1990
Předmět:
Zdroj: Journal of Neuroscience Research. 25:172-179
ISSN: 1097-4547
0360-4012
Popis: Previous work (Maxwell and Forbes: Development 101:767–776, 1987) has shown that an overlay of reconstituted basement membrane-like (RBM) gel dramatically increased the number of catecholaminepositive (CA+) cells which differentiated in neural crest cultures. We report here that this increase was inhibited when cultures were grown for 7 days in the presence of agents that elevate cAMP, such as 8-bromo-cAMP and 3-isobutyl-1-methylxanthine. The action of 8-bromo-cAMP was dose dependent with a half-maximal effect at about 50 μM. The development of CA+ cells was dramatically reduced when 8-bromo-cAMP was present from days 0–4 in vitro, but was relatively unaffected if 8-bromo-cAMP was present from days 4–7 in vitro. The development of tyrosine hydroxylase immunoreactive cells was also inhibited by 8-bromo-cAMP. The addition of 8-bromo-cAMP increased the number of melanocytes and resulted in either no change or only modest reductions in the number of E/C8 and neurofilament immunoreactive cells, indiciting that the effect on CA+ cell ontogeny was selective. In contrast to the effectof 8-bromo-cAMP, addition of 8-bromo-cGMP did not inhibit CA+ cell development in the presence of the RBM gel nor did it stimulate CA+ cell development in the absence of the RBM gel overlay. Our results suggest that cAMP may be an important regulator of phenotypic expression in at least some neural crest cell lineages. In particular, these observations provide evidence that agents that elevate cAMP levels can act to prevent an essential part of the normal cellular response leading to the normal cellular response leading to the appearance of CA+ phenotype in neural crest cultures.
Databáze: OpenAIRE
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