Tailoring a Combination Preerythrocytic Malaria Vaccine
| Autor: | Karolis Bauza, Tomas Malinauskas, Arturo Reyes-Sandoval, Erwan Atcheson, Andrew M. Blagborough |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
animal structures Erythrocytes viruses Immunology Genetic Vectors Plasmodium falciparum Protozoan Proteins Antibodies Protozoan Vaccinia virus Microbiology complex mixtures Viral vector 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine parasitic diseases Malaria Vaccines medicine Animals Humans Plasmodium berghei Malaria Falciparum Mice Inbred ICR biology Malaria vaccine fungi Antibody titer 11 Medical And Health Sciences 06 Biological Sciences medicine.disease biology.organism_classification Virology 3. Good health Circumsporozoite protein Mice Inbred C57BL 030104 developmental biology Infectious Diseases chemistry Microbial Immunity and Vaccines Parasitology Female 07 Agricultural And Veterinary Sciences Vaccinia Malaria 030215 immunology |
| Zdroj: | Infection and Immunity |
| ISSN: | 1098-5522 0019-9567 |
| Popis: | The leading malaria vaccine candidate, RTS,S, based on the Plasmodium falciparum circumsporozoite protein (CSP), will likely be the first publicly adopted malaria vaccine. However, this and other subunit vaccines, such as virus-vectored thrombospondin-related adhesive protein (TRAP), provide only intermediate to low levels of protection. In this study, the Plasmodium berghei homologues of antigens CSP and TRAP are combined. TRAP is delivered using adenovirus- and vaccinia virus-based vectors in a prime-boost regime. Initially, CSP is also delivered using these viral vectors; however, a reduction of anti-CSP antibodies is seen when combined with virus-vectored TRAP, and the combination is no more protective than either subunit vaccine alone. Using an adenovirus-CSP prime, protein-CSP boost regime, however, increases anti-CSP antibody titers by an order of magnitude, which is maintained when combined with virus-vectored TRAP. This combination regime using protein CSP provided 100% protection in C57BL/6 mice compared to no protection using virus-vectored TRAP alone and 40% protection using adenovirus-CSP prime and protein-CSP boost alone. This suggests that a combination of CSP and TRAP subunit vaccines could enhance protection against malaria. |
| Databáze: | OpenAIRE |
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