CRISPR-based DNA and RNA detection with liquid-liquid phase separation
| Autor: | Anna C. Haagsma, Stan J. J. Brouns, Rodrigo Gonzalez-Linares, Franklin L. Nobrega, Jeroen M. Jacques, Timon Idema, Willem Kasper Spoelstra, Marileen Dogterom, Dimphna H. Meijer, Louis Reese |
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| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
Biophysics Nucleic acid sequence RNA Computational biology DNA Articles Molecular diagnostics Endonucleases 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine chemistry Polynucleotide Cleave Nucleic acid CRISPR Clustered Regularly Interspaced Short Palindromic Repeats CRISPR-Cas Systems 030217 neurology & neurosurgery 030304 developmental biology |
| Zdroj: | Biophys J |
| ISSN: | 1542-0086 |
| Popis: | The ability to detect specific nucleic acid sequences allows for a wide range of applications such as the identification of pathogens, clinical diagnostics, and genotyping. CRISPR-Cas proteins Cas12a and Cas13a are RNA-guided endonucleases that bind and cleave specific DNA and RNA sequences, respectively. After recognition of a target sequence, both enzymes activate indiscriminate nucleic acid cleavage, which has been exploited for sequence-specific molecular diagnostics of nucleic acids. Here, we present a label-free detection approach that uses a readout based on solution turbidity caused by liquid-liquid phase separation (LLPS). Our approach relies on the fact that the LLPS of oppositely charged polymers requires polymers to be longer than a critical length. This length dependence is predicted by the Voorn-Overbeek model, which we describe in detail and validate experimentally in mixtures of polynucleotides and polycations. We show that the turbidity resulting from LLPS can be used to detect the presence of specific nucleic acid sequences by employing the programmable CRISPR-nucleases Cas12a and Cas13a. Because LLPS of polynucleotides and polycations causes solutions to become turbid, the detection of specific nucleic acid sequences can be observed with the naked eye. We furthermore demonstrate that there is an optimal polynucleotide concentration for detection. Finally, we provide a theoretical prediction that hints towards possible improvements of an LLPS-based detection assay. The deployment of LLPS complements CRISPR-based molecular diagnostic applications and facilitates easy and low-cost nucleotide sequence detection. |
| Databáze: | OpenAIRE |
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